Anti-PPAP2B Rabbit Polyclonal Antibody for WB, ELISA - O14495

Core Product Specifications and Parameters

Biological Background: PPAP2B Function and Localization

• PPAP2B, also known as phospholipid phosphatase 3 (PLPP3) or PAP2-beta, is a magnesium-independent lipid phosphate phosphohydrolase encoded by the PLPP3 gene in humans.

• It catalyzes the dephosphorylation of diverse bioactive lipid phosphate esters including phosphatidate (PA), lysophosphatidate (LPA), diacylglycerol pyrophosphate (DGPP), sphingosine 1-phosphate (S1P), and ceramide 1-phosphate (C1P), as well as N-oleoyl ethanolamine phosphate. Related references: PMID:27694435, PMID:9607309, PMID:9705349

• The enzyme exhibits both extracellular and intracellular phosphatase activities, enabling hydrolysis and cellular uptake of lipid mediators from the extracellular milieu, thereby regulating signal transduction. Related references: PMID:23591818, PMID:27694435, PMID:9607309

• Through dephosphorylation of extracellular S1P, PPAP2B plays a critical role in vascular homeostasis, modulating endothelial cell migration, adhesion, survival, proliferation, and pro-inflammatory cytokine production. Related references: PMID:27694435

• Independently of its phosphatase activity, PPAP2B participates in the Wnt signaling pathway and stabilization of beta-catenin, influencing cell proliferation, migration, and differentiation in angiogenesis and tumor growth, and also plays a role in integrin-mediated cell-cell adhesion. Related references: PMID:20123964, PMID:21569306, PMID:12660161, PMID:16099422

• Subcellularly, PPAP2B localizes to the plasma membrane, basolateral cell membrane, endoplasmic reticulum, Golgi apparatus, and membrane rafts, reflecting its dynamic trafficking and function in lipid signaling.

• PPAP2B is ubiquitously expressed, with highest levels in heart and placenta. Related references: PMID:12660161, PMID:9305923

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide corresponding to the N-terminal region (amino acids 1-100) of human PPAP2B. Consider that the antibody may recognize endogenous PPAP2B across human, mouse, and rat due to high sequence homology in this region.
• In Western blot (WB), PPAP2B is expected to migrate at a molecular weight of approximately 35 kDa. Validate the antibody using positive control lysates from tissues with high expression such as heart or placenta.
• For ELISA, the antibody can be used for detection; optimal working dilution and coating conditions should be determined empirically.
• As a polyclonal antibody, lot-to-lot consistency should be monitored; consider running a standard positive control sample in each experiment.

CamelBio: Your One-Stop Sourcing Bridge

At CamelBio, we serve as a comprehensive sourcing partner for IVD raw materials, offering ready-to-use and custom solutions for antibody-based research and diagnostic development. Our portfolio includes validated antibody pairs, optimized polyclonal and monoclonal antibodies, and ancillary reagents, enabling streamlined procurement for projects involving lipid phosphatase targets like PPAP2B. Let us assist you in securing high-quality components from early-stage concept to clinical application.