Anti-PPAP2A Polyclonal Antibody for WB, ELISA - O14494

Core Product Specifications and Parameters

Biological Background: Phospholipid Phosphatase 1 (PPAP2A) Function and Localization

• PPAP2A encodes phospholipid phosphatase 1 (PLPP1), also known as PAP2-alpha, a magnesium-independent lipid phosphate phosphohydrolase.
• Catalyzes dephosphorylation of various bioactive lipid phosphates including phosphatidate, lysophosphatidate, sphingosine 1-phosphate, and ceramide 1-phosphate. Related references: PMID:10962286 PMID:17379599 PMID:9305923
• Acts on N-oleoyl ethanolamine phosphate, a potential physiological compound. Related references: PMID:9607309
• Functions at the plasma membrane to hydrolyze extracellular lipid mediators, facilitating their cellular uptake and regulating signal transduction. Related references: PMID:10962286 PMID:12909631 PMID:15461590
• Essential for extracellular S1P hydrolysis and subsequent intracellular S1P generation. Related references: PMID:17379599
• Involved in regulation of inflammation, platelet activation, cell proliferation, and migration. Related references: PMID:12909631 PMID:15461590
• Localized to the cell membrane, apical membrane, membrane rafts, and caveolae.
• Widely expressed with highest levels in prostate; down-regulated in colon adenocarcinomas. Related references: PMID:9305923 PMID:9570154

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide from the N-terminus (aa 1-100). Verify sequence conservation across species and isoforms if cross-reactivity or isoform specificity is critical.
• For Western blotting, the predicted molecular weight is ~32 kDa, but glycosylation may increase the apparent mass. Include appropriate molecular weight markers and positive controls.
• For ELISA, consider pairing with a detection antibody that recognizes a non-overlapping epitope. Optimize coating concentration and blocking conditions for your specific assay format.
• This antibody is supplied as a rabbit polyclonal; lot-to-lot consistency should be confirmed for long-term studies.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio enables diagnostic manufacturers and research teams to efficiently source IVD raw materials and technical expertise. This anti-PPAP2A polyclonal antibody exemplifies the high-quality reagents available through our supply network, primarily supporting studies in lipid signaling and phosphatase research. From validated antibody pairs to bulk ancillary reagents, our one-stop platform simplifies procurement and accelerates your project from concept to clinic.