Anti-PPAN Rabbit Polyclonal Antibody for WB, IHC-P, IF/ICC, ELISA - Q9NQ55

Core Product Specifications and Parameters

Biological Background: PPAN Function and Localization

• PPAN (Suppressor of SWI4 1 homolog, also known as Brix domain-containing protein 3 or Peter Pan homolog) is encoded by the PPAN gene (alias BXDC3, SSF1).
• It localizes to the nucleus, specifically the nucleolus, suggesting involvement in ribosome biogenesis or nucleolar processes.
• The protein is widely expressed across human tissues.
• May have a role in cell growth, though its precise molecular function is not fully characterized.
• PPAN undergoes phosphorylation and acetylation, indicating regulation by post-translational modifications.
• Alternative splicing generates multiple transcript variants, potentially yielding protein isoforms with distinct properties.
• The three-dimensional structure of PPAN has been solved, contributing to structural biology databases.
• It is part of the human reference proteome, reflecting its significance in proteomic studies.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the N-terminal region (amino acids 1-300) of human PPAN. This region may target conserved functional domains; consider validating specificity if cross-reactivity with mouse is critical.
• For western blotting (WB), the predicted molecular weight is approximately 53 kDa. Verify the band size in your sample system, as post-translational modifications or alternative splicing may shift migration.
• In immunofluorescence (IF/ICC), anticipate nucleolar staining. Appropriate fixation and permeabilization conditions should be optimized to preserve nucleolar morphology.
• For immunohistochemistry (IHC-P), test antigen retrieval methods on positive control tissues known to express PPAN (e.g., widely expressed tissues).
• The antibody is recommended for ELISA; however, appropriate coating and detection conditions should be determined experimentally.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is a dedicated IVD raw-material partner providing one-stop access to validated antibodies, bulk reagents, and custom sourcing for diagnostic development. Complementing this anti-PPAN polyclonal antibody—ideal for studying cell growth and nucleolar biology—we offer optimized monoclonal and polyclonal formats, matched antibody pairs, and ancillary reagents to accelerate your assay development and scale-up. Our team can also source rare or emerging targets, ensuring a seamless supply chain from concept to clinic.