Anti-PPA1 Polyclonal Antibody for WB, ELISA - Q15181

Core Product Specifications and Parameters

Biological Background: PPA1 Function and Localization

• Inorganic pyrophosphatase (PPA1), also known as PPase, PP, PP1, IOPPP, HEL-S-66p, or SID6-8061, is a ubiquitously expressed enzyme that catalyzes the hydrolysis of inorganic diphosphate (PPi) into two orthophosphate molecules, driving biosynthetic reactions and energy metabolism. Related references: PMID:33036755, PMID:38428647
• Exhibits MAP kinase serine/threonine phosphatase activity toward c-Jun N-terminal kinase (JNK), and in neuronal cells, negatively regulates neurite growth via JNK dephosphorylation.
• Predominantly localized to the cytoplasm, consistent with its role in cellular phosphate homeostasis.
• Member of the inorganic pyrophosphatase family, conserved across eukaryotes and required for multiple biosynthetic pathways.
• Contains acetylation and phosphorylation sites, indicating potential regulation by post-translational modifications.
• Requires magnesium ions as a cofactor for catalytic activity.

Experimental Guidance and Technical Tips

• For Western blot, use whole-cell lysates from human, mouse, or rat tissues/cell lines. The expected band size is approximately 33 kDa.
• In ELISA applications, the immunogen is a full-length recombinant protein; consider optimizing coating conditions and antibody titration for your specific assay format.
• Since PPA1 is widely expressed, validate specificity by including appropriate negative controls (e.g., knockdown lysates or blocking peptide) if off-target binding is a concern.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio connects diagnostic manufacturers and research laboratories with a comprehensive portfolio of IVD raw materials, including validated antibody pairs, optimized monoclonal and polyclonal antibodies, and bulk ancillary reagents. For targets like PPA1, involved in phosphate metabolism and neuronal signaling, our supply platform supports your assay development with reliable sourcing solutions. From concept to clinic, we provide a one-stop bridge to accelerate your diagnostic and research workflows.