Anti-PPA1 Polyclonal Antibody for WB, ELISA - Q15181

Core Product Specifications and Parameters

Biological Background: PPA1 Function and Localization

• PPA1 encodes inorganic pyrophosphatase (PPase), a ubiquitously expressed enzyme that catalyzes the hydrolysis of inorganic diphosphate into two phosphate molecules, driving biosynthetic reactions and energy metabolism. Related references: PMID:33036755 PMID:38428647
• In addition to its pyrophosphatase activity, PPA1 displays MAP kinase serine/threonine phosphatase activity toward c-Jun N-terminal kinase (JNK); in neuronal cells, it negatively regulates neurite growth via JNK dephosphorylation (By similarity).
• The PPA1 gene belongs to the PPase family and is also known by aliases PP, PP1, IOPPP, HEL-S-66p, and SID6-8061.
• PPA1 is primarily localized in the cytoplasm, consistent with its role in intracellular phosphate metabolism.
• The protein is subject to acetylation and phosphorylation, and it binds magnesium ions as essential cofactors, as annotated in UniProt keywords.
• Expression of PPA1 is widespread across tissues, underscoring its housekeeping functions.

Experimental Guidance and Technical Tips

• The immunogen used is a recombinant fusion protein spanning amino acids 1-289 (near full-length) of human PPA1 (NP_066952.1), which is expected to generate polyclonal antibodies recognizing both native and denatured antigen. Consider this when planning antigen retrieval or native detection assays.
• For Western blot (WB), the predicted molecular weight of human PPA1 is approximately 33 kDa; validate detection in whole-cell lysates from human, mouse, or rat samples. The antibody has been reported to cross-react with these species.
• In ELISA applications, this polyclonal preparation may serve as a capture or detection reagent. Titration and pairing with a suitable monoclonal antibody or conjugate are recommended to optimize signal-to-noise.
• As with any polyclonal antibody, lot-to-lot variability can occur; independent re-validation in your assay system is advised.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio specializes in providing diagnostic manufacturers and research laboratories with a comprehensive range of IVD raw materials, technical services, and consultative support—from concept to clinic. For targets like PPA1, our portfolio includes validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and custom sourcing for rare‑target raw materials. Whether you need a single reagent or an entire assay development pipeline, we are your trusted procurement partner.