Anti-PP2A Catalytic β (PPP2CB) Rabbit Polyclonal Antibody for WB, IHC-P, IF/ICC, ELISA - P62714

Core Product Specifications and Parameters

Biological Background: PP2A Catalytic β Function and Localization

• PP2A Catalytic β (PPP2CB) is the catalytic subunit of serine/threonine-protein phosphatase 2A (PP2A), an enzyme that regulates diverse cellular processes by dephosphorylating key phosphoproteins (Probable).
• PP2A functions in modulating the activity of enzymes such as phosphorylase B kinase, casein kinase 2, mitogen-stimulated S6 kinase, and MAP-2 kinase, thereby influencing signal transduction pathways.
• As part of the striatin-interacting phosphatase and kinase (STRIPAK) complexes, PPP2CB contributes to the regulation of critical signaling pathways including Hippo, MAPK, nuclear receptor, and cytoskeleton remodeling. Related references: PMID:18782753
• STRIPAK complexes are involved in various biological processes like cell growth, differentiation, apoptosis, metabolism, and immune regulation.
• The protein localizes to multiple subcellular compartments: cytoplasm, nucleus, centromeres, and cytoskeleton (spindle pole), supporting its broad regulatory roles.
• PPP2CB is classified as a hydrolase and belongs to the PPP family of protein phosphatases; it requires divalent metal cations (manganese) for activity and undergoes post-translational modifications such as methylation and ubiquitination.

Experimental Guidance and Technical Tips

• The immunogen is a recombinant fusion protein covering amino acids 1-309 (full-length) of human PP2A Catalytic β, which should generate antibodies recognizing linear and conformational epitopes across the entire protein.
• For Western blot (WB), consider using a starting dilution of 1:500-1:2000 and validate with appropriate positive controls (e.g., HeLa or HEK293 whole cell lysates) given the predicted molecular weight of ~36 kDa.
• In immunohistochemistry (IHC-P) and immunofluorescence (IF/ICC), optimize antigen retrieval and antibody concentration; note the reported localization in cytoplasm, nucleus, and spindle poles.
• This antibody demonstrates cross-reactivity with mouse and rat PPP2CB, making it suitable for common rodent model studies; validate species-specific reactivity in your experimental system.
• For ELISA applications, pair with a matched detection system and confirm specificity using recombinant protein or peptide blocking.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers a comprehensive one-stop sourcing platform for IVD raw materials, supporting diagnostic manufacturers and research labs with validated antibody reagents. This anti-PP2A Catalytic β rabbit polyclonal antibody exemplifies our commitment to providing high-quality tools for studying protein phosphorylation and signal transduction. Beyond individual antibodies, we supply optimized antibody pairs, bulk monoclonal/polyclonal antibodies, and ancillary reagents to accelerate your assay development from concept to clinic.