Anti-PP2A Catalytic α Rabbit Polyclonal Antibody for WB, IHC-P, IF/ICC - P67775

Core Product Specifications and Parameters

Biological Background: PP2A Catalytic α Function and Localization

• Serine/threonine-protein phosphatase 2A catalytic subunit alpha isoform (PP2A-Cα) is the major catalytic subunit of PP2A holoenzymes, regulating diverse cellular processes including signal transduction, cell cycle progression, and transcription (PubMed:10801873, 12473674, 17245430, 22613722).
  Related references: PMID:10801873, PMID:12473674, PMID:17245430, PMID:22613722
• It dephosphorylates key substrates such as WEE1 (promoting G2/M checkpoint; PubMed:33108758), MYC (targeting it for degradation; PubMed:25438055), FOXO3 (stabilizing it; PubMed:30513302), and PIM3 (triggering its turnover; PubMed:12473674).
  Related references: PMID:33108758, PMID:25438055, PMID:30513302, PMID:12473674
• PP2A-Cα functions within the STRIPAK complex to integrate phosphatase and kinase signals controlling Hippo, MAPK, and cytoskeleton remodeling pathways (PubMed:33633399).
  Related references: PMID:33633399
• As part of the INTAC complex, PP2A-Cα enforces a transcriptional quality checkpoint by dephosphorylating RNA polymerase II CTD and SPT5, causing premature termination of unfavorably configured transcripts (PubMed:33243860, 34004147, 37080207).
  Related references: PMID:33243860, PMID:34004147, PMID:37080207
• Localizes to the cytoplasm, nucleus, centromeres, and spindle poles, consistent with its roles in mitosis, meiosis, and transcription regulation.
• Post-translational modifications include reversible methylation of the C‑terminal leucine, which is critical for holoenzyme assembly, as well as phosphorylation and ubiquitination.
• Alternative splicing generates multiple isoforms; mutations in PPP2CA are linked to intellectual disability, underscoring its essential role in neurodevelopment.
• Contains iron, manganese, and zinc metal-binding sites; classified as a hydrolase and phosphoprotein phosphatase involved in a wide range of cellular signaling events.

Experimental Guidance and Technical Tips

• The immunogen encompasses the N‑terminal 309 amino acids covering the catalytic domain; consider validating this antibody in your specific sample matrix and application.
• Recommended for WB, IHC‑P, IF/ICC, IP, and ELISA—optimize sample preparation, blocking conditions, and detection reagents for each technique.
• High sequence conservation among human, mouse, and rat suggests cross-reactivity; confirm reactivity in your experimental system with appropriate controls.
• For IP and IF/ICC, test different antibody concentrations to achieve optimal signal-to-noise ratios.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your one-stop partner for IVD raw material sourcing, supporting diagnostic manufacturers and researchers from concept to clinic. This PP2A Catalytic α antibody exemplifies our commitment to providing high-quality, validated reagents for signal transduction and cancer research. In addition to purified antibodies, CamelBio offers recombinant proteins, antibody pairs, bulk ancillary reagents, and customized rare-target procurement to accelerate your assay development.