Anti-PP2A-B56β/PR61β/PPP2R5B Polyclonal Antibody for WB and ELISA - Q15173

Core Product Specifications and Parameters

Biological Background: PP2A-B56β/PR61β/PPP2R5B Function and Localization

• PP2A-B56β, also known as PR61β or B56beta, is a regulatory subunit of the serine/threonine-protein phosphatase 2A (PP2A) holoenzyme, encoded by the PPP2R5B gene. It is alternatively spliced, producing multiple isoforms (UniProt Q15173).
• As a regulatory B subunit, it directs PP2A substrate specificity, subcellular localization, and responsiveness to phosphorylation, thereby tuning phosphatase activity toward specific targets.
• The phosphorylated form of PP2A-B56β mediates the interaction between PP2A and AKT1, facilitating the dephosphorylation and inactivation of AKT1, a key node in cell survival and growth signaling.
• PP2A-B56β belongs to the B56/PR61 family of PP2A regulatory subunits, which are critical for diverse cellular processes including cell cycle control, apoptosis, and signal transduction.
• The protein is predominantly localized in the cytoplasm, consistent with its role in modulating cytosolic signaling pathways.
• Post-translational modifications include phosphorylation (it is a phosphoprotein) and potential ubiquitination (Ubl conjugation), which may regulate its stability and interactions.
• Highest expression is observed in the brain, suggesting specialized functions in neuronal signaling, though it is widely expressed in other tissues.

Experimental Guidance and Technical Tips

• The immunogen corresponds to a C-terminal region (aa 400-497) of human PP2A-B56β. This region is conserved in rat (based on the cross-reactivity claim), enabling detection across species.
• For Western blot (WB), the expected molecular weight is approximately 57 kDa. Include appropriate positive and negative controls, and validate antibody specificity in your sample type, as background may vary.
• For ELISA, consider optimizing coating conditions and antibody dilutions. The antibody may be suitable for indirect ELISA using recombinant protein or cell lysates.
• Given that PP2A-B56β is a regulatory subunit, co-immunoprecipitation (IP) experiments may be possible to study PP2A complex assembly, but this has not been validated for this antibody.
• Because the protein expression is highest in brain, tissue lysates from brain (human, rat) may serve as strong positive controls for WB.

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As a dedicated IVD raw-material sourcing partner, CamelBio simplifies procurement of high-quality antibodies like this anti-PP2A-B56β polyclonal antibody for phosphatase research and signal transduction studies. We offer one-stop access to validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and can assist with sourcing rare-target raw materials to accelerate your diagnostic development from concept to clinic.