Anti-PP2A-B56γ/PR61γ/PPP2R5C Rabbit Polyclonal Antibody for WB, ELISA - Q13362

Core Product Specifications and Parameters

Biological Background: PP2A-B56γ/PR61γ/PPP2R5C Function and Localization

• PP2A-B56γ (PPP2R5C) is the gamma isoform of the 56 kDa regulatory B subunit of serine/threonine-protein phosphatase 2A.
• The B subunit modulates substrate selectivity and catalytic activity, and directs localization to specific subcellular compartments.
• As part of the PP2A holoenzyme, it may specifically dephosphorylate and activate TP53, playing a role in DNA damage-induced inhibition of cell proliferation.
• It also regulates the ERK signaling pathway through ERK dephosphorylation.
• Located in the nucleus, with specific association to chromosomes and centromeres.
• Highest expression in heart, skeletal muscle, and brain, with lower levels in pancreas, kidney, lung, and placenta.
• Post-translational modifications include phosphorylation and acetylation; also subject to alternative splicing.
• Associated with disease variants linked to intellectual disability (based on UniProt keywords).

Experimental Guidance and Technical Tips

• The immunogen corresponds to the N-terminal region (amino acids 1–320); the antibody may recognize epitopes within this domain.
• For Western blotting, optimize primary antibody dilution in the relevant species and tissue type; a typical starting range is 1:500–1:2000.
• For ELISA, validate the antibody as a capture or detection reagent using appropriate antigen standards and blocking conditions.
• Cross-reactivity with human, mouse, and rat enables multi-species studies; always confirm specificity using positive and negative controls.
• As a polyclonal preparation, it may detect multiple isoforms or degradation products; consider siRNA knockdown or recombinant protein controls.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides comprehensive one-stop access to IVD raw materials, technical services, and consulting—from concept to clinic. This anti-PPP2R5C antibody supports cell signaling and phosphatase research, complementing our portfolio of optimized monoclonal and polyclonal antibodies, validated antibody pairs, bulk ancillary reagents, and rare-target sourcing. Contact us to tailor procurement solutions for your diagnostic or research programs.