Anti-PP2A-B56δ/PR61δ/PPP2R5E Polyclonal Antibody for WB, ELISA - Q16537

Core Product Specifications and Parameters

Biological Background: PP2A-B56δ/PR61δ/PPP2R5E Function and Localization

• The serine/threonine-protein phosphatase 2A 56 kDa regulatory subunit epsilon isoform (PPP2R5E) is a regulatory B subunit of the heterotrimeric PP2A holoenzyme, designated B56δ/PR61δ. It is also known as PP2A B subunit isoform B'-epsilon or B56-epsilon.
• The B regulatory subunit is proposed to modulate substrate selectivity and catalytic activity of the PP2A core enzyme, thereby influencing dephosphorylation of various targets involved in cell signaling, cell cycle, and apoptosis.
• It belongs to the B56 (PR61) family of regulatory subunits, which are distinguished by a conserved central domain and variable N- and C-termini, and they often provide subcellular targeting cues.
• The protein localizes predominantly to the cytoplasm, as indicated by UniProt annotation, suggesting a role in cytosolic dephosphorylation events.
• Post-translational modifications include acetylation and phosphorylation (annotated from large-scale proteomics), and the gene undergoes alternative splicing, potentially generating multiple isoforms.
• Keywords: 3D-structure, Acetylation, Alternative splicing, Cytoplasm, Direct protein sequencing, Phosphoprotein, Proteomics identification, Reference proteome.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide mapping to residues 50–150 (based on NP_006237.1). Consider aligning this region across species if cross-reactivity with orthologs is desired; the product already shows reactivity with rat.
• For western blotting, the predicted molecular weight is ~55 kDa. Bands of this size should be observed; additional bands may arise from alternative isoforms or post-translational modifications—validate using appropriate controls or siRNA knockdown in the relevant cell line.
• In ELISA, use the antibody as a capture or detection reagent as per standard indirect ELISA protocols. Workflow optimization may require titration of the antibody and antigen.
• The antibody is raised in rabbit, so secondary anti-rabbit IgG conjugates are required for detection. Ensure that blocking buffers and wash conditions are optimized to minimize high background.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your dedicated partner for IVD raw-material sourcing, providing validated antibody pairs, monoclonal and polyclonal antibodies, and bulk ancillary reagents for diagnostic assay development. This PP2A-B56δ polyclonal antibody is suitable for research in phosphatase signaling and cancer biology, and we can support your program with complementary raw materials, technical consultation, and scalable supply. From concept to clinic, CamelBio streamlines procurement so you can focus on innovation.