Anti-PP2A-B56δ/PR61δ/PPP2R5D Rabbit Monoclonal Antibody for WB, IHC-P, ELISA - Q14738

Core Product Specifications and Parameters

Biological Background: PP2A-B56δ Function and Localization

• PP2A-B56δ (PPP2R5D) is the delta isoform of the B56 regulatory subunit family of protein phosphatase 2A (PP2A). Other aliases include B56D, MRD35, and PR61δ.
• The B regulatory subunit modulates substrate selectivity and catalytic activity of the PP2A holoenzyme, and may direct localization to specific subcellular compartments.
• Subcellular localization: found in both cytoplasm and nucleus.
• Tissue specificity: Isoform Delta-2 is widely expressed, while Isoform Delta-1 is highly expressed in brain.
• Keywords from UniProt: alternative splicing (generating isoforms Delta-1 and Delta-2), phosphoprotein, disease variant (intellectual disability), and repeat domains. Mutations in PPP2R5D are associated with intellectual disability (MRD35).
• The protein has a predicted molecular weight of approximately 70 kDa (69992 Da).

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 480-602 at the C‑terminus of human PPP2R5D, which may recognize both Delta‑1 and Delta‑2 isoforms if the sequence is conserved. Validate isoform detection in the relevant sample system.
• For Western blotting, a band of ~70 kDa is expected; consider using appropriate positive (e.g., HeLa, HEK293) and negative controls.
• In immunohistochemistry (IHC‑P), antigen retrieval conditions should be optimized for the specific tissue and fixation method used.
• For ELISA applications, pairing with a capture/detection antibody pair may require testing for sensitivity and specificity in the intended matrix.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers diagnostic manufacturers and research institutes a streamlined, one-stop channel for procuring IVD raw materials—from validated antibody pairs to optimized monoclonal and polyclonal antibodies—alongside technical support that spans early development through clinical implementation. For research into protein phosphatase 2A regulation and signaling pathways, we supply bulk ancillary reagents and assist with rare‑target sourcing, ensuring your workflow stays fully supported.