Anti-PP2A-B56δ/PR61δ/PPP2R5D Polyclonal Antibody for WB, IHC-P, IF/ICC, ELISA - Q14738

Core Product Specifications and Parameters

Biological Background: PP2A-B56δ/PR61δ/PPP2R5D Function and Localization

• PP2A-B56δ (also known as PR61δ, B56delta, PPP2R5D) is a regulatory subunit of serine/threonine-protein phosphatase 2A (PP2A), playing a critical role in modulating substrate selectivity and catalytic activity.
• The protein directs the localization of the catalytic enzyme to specific subcellular compartments, including the cytoplasm and nucleus, influencing dephosphorylation events in these locations.
• Alternative splicing produces multiple isoforms; isoform Delta-2 is widely expressed while isoform Delta-1 is highly expressed in the brain, suggesting tissue-specific regulatory roles.
• As a phosphoprotein, PP2A-B56δ itself may be regulated by phosphorylation, and it participates in signaling networks governing cell growth and division.
• Keywords from UniProt indicate involvement in disease variants (intellectual disability) and highlight its importance in neurological function.
• The protein contains repeat regions characteristic of its family, which mediate protein-protein interactions essential for PP2A holoenzyme assembly.

Experimental Guidance and Technical Tips

• The immunogen corresponds to a synthetic peptide within amino acids 450-550 of human PPP2R5D, which may recognize both Delta-1 and Delta-2 isoforms; verify specificity in your system using isoform-specific controls.
• For Western blot (WB), the predicted molecular weight is ~70 kDa. Use appropriate gel percentage (e.g., 8-10% SDS-PAGE) and validate with positive control lysates from human, mouse, or rat tissues/cells.
• For immunohistochemistry (IHC-P), consider heat-induced antigen retrieval and test antibody dilutions starting at 1:50-1:200. Include appropriate negative controls (e.g., tissue known to lack target).
• For immunofluorescence (IF/ICC), permeabilize cells adequately and co-stain with organelle markers to confirm expected cytoplasmic/nuclear localization.
• For ELISA, the antibody can be used as a capture or detection reagent; optimize coating conditions and antibody concentrations for your specific assay format.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your dedicated one-stop IVD raw-material sourcing partner, supporting diagnostic manufacturers and research institutes from concept to clinic. For studies on phosphatase signaling and regulatory subunits like PPP2R5D, we supply validated antibody pairs, optimized polyclonal antibodies, and bulk ancillary reagents. Our expert team also assists with rare target raw-material sourcing, ensuring reliable supply for your projects.