Anti-PP2A-B55α/PR55α/PPP2R2A Rabbit Polyclonal Antibody for WB, IHC-P, ELISA - P63151

Core Product Specifications and Parameters

Biological Background: PP2A-B55α/PR55α/PPP2R2A Function and Localization

• PP2A-B55α (also known as PPP2R2A, B55 alpha, PR55 alpha) is a regulatory subunit of serine/threonine-protein phosphatase 2A (PP2A), responsible for substrate recognition.
• It plays a pivotal role in cell cycle regulation by controlling mitosis entry and exit through dephosphorylation of key substrates.
  Related references: PMID:1849734 PMID:33108758
• Mediates dephosphorylation of WEE1, preventing its ubiquitin-mediated degradation, increasing WEE1 protein levels, and promoting the G2/M checkpoint.
  Related references: PMID:33108758
• Involved in chromosome clustering during late mitosis by mediating dephosphorylation of MKI67 (Ki-67).
• Expressed ubiquitously across all tissues examined.
• Contains WD repeat domains, characteristic of the phosphatase 2A regulatory subunit B family.
• Subject to acetylation and alternative splicing, generating multiple isoforms.

Experimental Guidance and Technical Tips

• The immunogen spans nearly the full-length protein (aa 1–447), suggesting the antibody may recognize multiple epitopes and is suitable for detecting denatured protein in WB.
• For IHC-P, consider optimizing antigen retrieval conditions and validating the antibody dilution in a tissue known to express PP2A-B55α.
• In ELISA, use the recombinant immunogen as a positive control to establish assay parameters.
• Given ubiquitous expression, include appropriate positive and negative control samples to confirm specificity.
• Cross-reactivity with mouse and rat is expected based on high sequence homology; validation in the relevant model system is recommended.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as your comprehensive partner for IVD raw-material sourcing, offering validated antibody pairs, optimized monoclonal and polyclonal antibodies, and bulk ancillary reagents. This anti-PP2A-B55α antibody supports research into cell cycle regulation and cancer biology. We also facilitate rare-target raw-material procurement and provide technical consultancy to accelerate your development from concept to clinic.