Anti-PP2A-B55α/PR55α/PPP2R2A Rabbit pAb - P63151

Core Product Specifications and Parameters

Biological Background: PP2A-B55α/PR55α/PPP2R2A Function and Localization

• Regulatory subunit of serine/threonine-protein phosphatase 2A (PP2A), encoded by the PPP2R2A gene; belongs to the B55 family and undergoes alternative splicing.
• Controls mitosis entry and exit as a substrate-recognition subunit of PP2A (PubMed:1849734, PubMed:33108758). Related references: PMID:1849734 PMID:33108758
• Involved in chromosome clustering during late mitosis by mediating dephosphorylation of MKI67 (By similarity).
• Essential for PP2A-mediated dephosphorylation of WEE1, preventing its ubiquitin-mediated proteolysis and promoting the G2/M checkpoint (PubMed:33108758). Related references: PMID:33108758
• Expressed ubiquitously across all tissues examined.
• Contains WD repeats; subject to acetylation and other post-translational modifications (Keywords: 3D-structure, Acetylation, Alternative splicing, Proteomics identification, Reference proteome, Repeat, WD repeat).

Experimental Guidance and Technical Tips

• The immunogen covers residues 100–200 of human PP2A-B55α, a region that may confer isoform specificity; verify sequence homology if cross-reactivity with other B subunits is a concern.
• For Western blot, the predicted molecular weight is around 52 kDa (UniProt: 51692 Da). Validate the band in a positive cell line expressing PPP2R2A.
• For ELISA, consider using the antibody as capture or detection reagent paired with another PP2A-B55α antibody; assay conditions and limits should be optimized experimentally.
• Always include appropriate positive and negative controls (e.g., PPP2R2A knockdown lysates) to confirm specificity.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, labs, and research institutes with one-stop access to IVD raw materials, technical services, and consulting—covering every stage from concept to clinic. This rabbit polyclonal antibody targets PP2A-B55α, a key regulator of the cell cycle, and is suited for research in oncology and signal transduction. Beyond individual antibodies, we can supply validated antibody pairs, bulk ancillary reagents, and support rare-target raw-material sourcing to simplify your supply chain and accelerate development.