Anti-PP2A-B55α/PR55α/PPP2R2A Monoclonal Antibody for WB, ELISA - P63151

Core Product Specifications and Parameters

Biological Background: PP2A-B55α/PR55α/PPP2R2A Function and Localization

• PP2A-B55α (PPP2R2A) is the B55-alpha regulatory subunit of serine/threonine-protein phosphatase 2A (PP2A), a major cellular phosphatase. It belongs to a family of regulatory subunits that dictate substrate specificity and subcellular targeting.
• This subunit plays a critical role in cell cycle regulation by controlling mitosis entry and exit. It is essential for PP2A-mediated dephosphorylation of key substrates during mitotic progression. Related references: PMID:1849734, PMID:33108758
• It is involved in chromosome clustering during late mitosis by mediating dephosphorylation of MKI67, a critical step for proper chromosome segregation.
• PP2A-B55α dephosphorylates WEE1, preventing its ubiquitin-mediated proteolysis. This stabilizes WEE1 protein levels and promotes the G2/M checkpoint, ensuring accurate cell cycle arrest in response to damage. Related references: PMID:33108758
• The protein is ubiquitously expressed across all human tissues, reflecting its fundamental role in cell division control.
• Post-translational modifications include acetylation, and the protein features multiple WD repeats that mediate protein-protein interactions. Alternative splicing generates additional isoforms.

Experimental Guidance and Technical Tips

• The immunogen covers amino acids 94-194 of human PP2A-B55α, a region likely exposed in both native and denatured conditions, making the antibody suitable for WB and ELISA as specified.
• For Western blotting, a band around 52kDa is expected. When using mouse samples, verify cross-reactivity as documented; consider loading appropriate positive controls.
• Optimal antibody dilutions should be determined empirically for each assay system. Include blocking and washing steps per standard protocols.
• This monoclonal antibody offers batch-to-batch consistency; however, as with all antibodies, performance should be validated in the relevant sample matrix and application context.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supports diagnostic manufacturers and researchers by providing a seamless supply chain for IVD raw materials. This anti-PP2A-B55α monoclonal antibody, targeting a key cell-cycle regulatory phosphatase, is available alongside comprehensive sourcing solutions—including validated antibody pairs, bulk reagents, and customized monoclonal/polyclonal antibody development. Whether you need ancillary raw materials or assistance scaling up assay production, CamelBio streamlines procurement from discovery to clinical application.