Anti-PP2A-Aβ/PR65β/PPP2R1B Polyclonal Antibody for WB, ELISA - P30154

Core Product Specifications and Parameters

Biological Background: PP2A-Aβ/PR65β/PPP2R1B Function and Localization

• Encoded by the PPP2R1B gene, PP2A-Aβ/PR65β is the beta isoform of the scaffold subunit A of serine/threonine-protein phosphatase 2A (PP2A), also known as PP2A subunit A isoform PR65-beta or R1-beta.
• Acts as a scaffolding molecule that coordinates the assembly of the PP2A catalytic subunit (C) and a variable regulatory B subunit to form the heterotrimeric PP2A holoenzyme.
• Belongs to the protein phosphatase 2A regulatory subunit A family; contains multiple HEAT repeats likely mediating protein-protein interactions.
• Exhibits alternative splicing, leading to multiple isoforms with potential functional diversity.
• Subject to post-translational acetylation, which may regulate its activity or stability.
• Annotated as a reference proteome component and identified through extensive proteomics studies.
• 3D structural information is available, aiding in understanding its interaction interfaces.
• The protein has a predicted molecular mass of approximately 66 kDa (UniProt), though multiple isoforms and post-translational modifications may result in observed bands at 52–73 kDa.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide from the C-terminal region (aa 502–601), so the antibody may recognize full-length and truncated isoforms containing this epitope. Validate specificity in the relevant sample system using appropriate controls.
• For Western blotting, multiple bands may be observed due to alternative splicing and post-translational modifications (expected molecular weights: 52, 61, 66, 73 kDa). Use freshly prepared lysates and consider phosphatase inhibitors to preserve modification states.
• ELISA suitability has been demonstrated; optimization of coating antigen and antibody dilution is recommended. Suitable for detecting target protein in human and rat samples.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your integrated sourcing partner for IVD raw materials, offering validated antibody pairs, optimized monoclonal/polyclonal antibodies, and bulk ancillary reagents to accelerate diagnostic development. For researchers studying protein phosphatase 2A (PP2A) signaling, we provide reliable access to this anti-PP2A-Aβ/PR65β/PPP2R1B rabbit polyclonal antibody along with technical consulting to support your assay development and biomarker research.