Anti-PP2A-Aα/PR65α/PPP2R1A Rabbit Polyclonal Antibody for WB, IF/ICC, ELISA - P30153

Core Product Specifications and Parameters

Biological Background: PP2A-Aα/PR65α/PPP2R1A Function and Localization

• PR65/PPP2R1A is the alpha isoform of the 65 kDa regulatory subunit A of serine/threonine-protein phosphatase 2A (PP2A), encoded by the gene PPP2R1A (alternative names: PP2AA, PR65A). It functions as a scaffolding molecule that coordinates the assembly of the catalytic subunit and a variable regulatory B subunit. Related references: PMID:15525651, PMID:16580887, PMID:33243860
• The protein is essential for proper chromosome segregation and centromeric localization of SGO1 during mitosis, ensuring genetic stability. Related references: PMID:16580887
• Through its scaffolding role, PR65/PPP2R1A is a key component of the STRIPAK (striatin-interacting phosphatase and kinase) complexes, which regulate signaling pathways including Hippo, MAPK, and nuclear receptor pathways, and are involved in cell growth, differentiation, and apoptosis. Related references: PMID:18782753, PMID:33633399
• As part of the Integrator-PP2A (INTAC) complex, it mediates a quality checkpoint during transcription elongation by promoting dephosphorylation of the C-terminal domain (CTD) of RNA polymerase II and the elongation factor SUPT5H/SPT5, leading to premature termination of unfavorably configured transcripts. Related references: PMID:33243860, PMID:34004147
• Together with the RACK1 adapter, PR65/PPP2R1A mediates dephosphorylation of AKT1 at Ser-473, preventing AKT1 activation and downstream mTOR signaling; this activity is critical for regulatory T-cell homeostasis.
• PR65/PPP2R1A localizes to multiple subcellular compartments including the cytoplasm, nucleus, centromeres, lateral cell membrane, and dendrites, reflecting its diverse functions.
• The protein contains HEAT repeats and is subject to acetylation; it is identified by direct protein sequencing and implicated in disease variants including intellectual disability, as well as host-virus interactions.
• Regulates the recruitment of the SKA complex to kinetochores, contributing to mitotic spindle assembly and chromosome alignment. Related references: PMID:28982702

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 1-270 of human PP2A-Aα/PR65α/PPP2R1A, a region that includes multiple HEAT repeats; antibody performance may be influenced by post-translational modifications or isoform-specific differences. Validate specificity in the relevant sample system.
• For Western blotting, an expected band around 65 kDa is observed in human, mouse, and rat cell lysates. Use appropriate controls and consider the protein's nuclear and cytoplasmic distribution.
• In immunofluorescence/ICC, punctate nuclear and centromeric signals may be observed; fixation and permeabilization conditions should be optimized to preserve epitope accessibility.
• ELISA applications may require coating with the recombinant immunogen or native protein; titration experiments are recommended to determine optimal antibody dilutions.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your reliable one-stop partner for IVD raw-material sourcing, offering a comprehensive portfolio of validated antibodies, recombinant proteins, and ancillary reagents. For research on PP2A signaling and chromosomal regulation, we provide optimized polyclonal antibodies like this anti-PP2A-Aα/PR65α/PPP2R1A rabbit pAb, along with bulk supplies, custom formulations, and assistance in accessing rare target raw materials to support your assay development and manufacturing needs.