Anti-PP1 beta Rabbit Polyclonal Antibody for WB, IHC-P, IF/ICC, ELISA - P62140

Core Product Specifications and Parameters

Biological Background: Serine/threonine-protein phosphatase PP1-beta catalytic subunit Function and Localization

• Also known as PP1beta, PP-1B, PPP1CD, and PPP1beta, this enzyme is encoded by the PPP1CB gene and belongs to the PP1 family of serine/threonine phosphatases.
• Functions as the catalytic subunit of protein phosphatase 1 (PP1) holoenzymes, which regulate diverse processes including cell division, glycogen metabolism, muscle contractility, and protein synthesis.
• Component of the PTW/PP1 phosphatase complex, which plays a critical role in the control of chromatin structure and the transition from mitosis to interphase.
• In concert with casein kinase I delta and epsilon (CSNK1D and CSNK1E), regulates circadian period length by modulating the phosphorylation dynamics of PER1 and PER2 proteins.
• Dephosphorylates FOXP3 at Ser-418 in regulatory T-cells from rheumatoid arthritis patients, leading to FOXP3 inactivation and Treg dysfunction. Related references: PMID:23396208
• Core component of the SHOC2-MRAS-PP1c (SMP) holophosphatase complex, which specifically dephosphorylates inhibitory sites on RAF1, BRAF, and ARAF kinases to activate the MAPK signaling pathway. Related references: PMID:35768504 PMID:35831509 PMID:36175670
• Localizes to the cytoplasm, nucleus, nucleoplasm, and nucleolus, reflecting its involvement in both cytoplasmic and nuclear signaling events.
• Post-translationally modified by acetylation and phosphorylation, and requires manganese as a cofactor for catalytic activity.

Experimental Guidance and Technical Tips

• The immunogen was a synthetic peptide from amino acids 227-327 of human PP1 beta. For Western blot (WB), expect a band at approximately 37 kDa; validate the antibody in your specific sample system.
• For immunohistochemistry (IHC-P) and immunofluorescence (IF/ICC), consider optimizing antigen retrieval and fixation/permeabilization protocols to ensure adequate epitope accessibility.
• As a rabbit polyclonal antibody, lot-to-lot consistency should be verified for critical quantitative assays; consider using appropriate positive and negative controls.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supports diagnostic manufacturers and researchers with a comprehensive portfolio of IVD raw materials, including validated antibody pairs, optimized polyclonal antibodies, and bulk ancillary reagents. This anti-PP1 beta antibody is well-suited for studies on cell cycle regulation, glycogen metabolism, and circadian rhythms. For specialized requirements or rare target sourcing, CamelBio offers tailored procurement solutions to accelerate your assay development from concept to clinic.