Anti-PP1 beta Rabbit Polyclonal Antibody for WB, IHC-P, IF/ICC, ELISA - P62140

Core Product Specifications and Parameters

Biological Background: PP1 beta Function and Localization

• Serine/threonine-protein phosphatase PP1-beta catalytic subunit encoded by the PPP1CB gene, a ~37 kDa member of the PPP family of protein phosphatases.
• Associates with over 200 regulatory proteins to form highly specific holoenzymes that dephosphorylate hundreds of biological targets, playing essential roles in cell division, glycogen metabolism, muscle contractility, and protein synthesis.
• Involved in the regulation of ionic conductances and long-term synaptic plasticity, highlighting its role in neuronal signaling.
• Component of the PTW/PP1 phosphatase complex, which controls chromatin structure and cell cycle progression during the transition from mitosis into interphase.
• In balance with casein kinase I isoforms CSNK1D and CSNK1E, determines circadian period length by regulating the speed and rhythmicity of PER1 and PER2 phosphorylation; may also dephosphorylate CSNK1D and CSNK1E.
• Dephosphorylates the 'Ser-418' residue of FOXP3 in regulatory T-cells (Treg) from rheumatoid arthritis patients, leading to FOXP3 inactivation and Treg dysfunction. Related references: PMID:23396208
• Core component of the SHOC2-MRAS-PP1c (SMP) holophosphatase complex, which dephosphorylates the inhibitory 'Ser-259' of RAF1, 'Ser-365' of BRAF and 'Ser-214' of ARAF, thereby stimulating MAPK pathway activity. Related references: PMID:35768504 PMID:35831509 PMID:36175670
• Subcellular localization includes cytoplasm, nucleus, nucleoplasm, and nucleolus; key post-translational modifications include acetylation and phosphorylation, and the protein is associated with biological rhythms, carbohydrate metabolism, and disease variants.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide corresponding to amino acids 227-327 of human PP1 beta (NP_002700.1); this region is well-conserved, enabling cross-reactivity with mouse and rat.
• For Western blotting (WB), a band at approximately 37 kDa is expected; consider using reducing conditions and appropriate loading controls.
• For immunohistochemistry (IHC-P) and immunofluorescence (IF/ICC), optimize antigen retrieval and permeabilization protocols as PP1 beta localizes to both cytoplasmic and nuclear compartments.
• The polyclonal nature of this antibody provides robust signal amplification; however, lot-to-lot consistency should be verified for quantitative assays.
• For ELISA applications, pairing with a suitable capture antibody or using direct coating may be required; validate the optimal antibody concentration.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers this anti-PP1 beta polyclonal antibody as a core raw material for diagnostic and research applications, supporting the development of assays related to cell cycle, signal transduction, and metabolic disorders. As a trusted one-stop sourcing partner, we supply validated antibody pairs, optimized monoclonal/polyclonal antibodies, and bulk ancillary reagents to streamline your IVD workflow from concept to clinic. For rare targets or custom requirements, our team can assist in sourcing specialized raw materials to meet your project needs.