Anti-PP1 beta Rabbit Monoclonal Antibody for WB, IHC-P, IF/ICC, ELISA - P62140

Core Product Specifications and Parameters

Biological Background: PP1 beta Function and Localization

• PP1 beta (encoded by PPP1CB) is the catalytic subunit of the serine/threonine-protein phosphatase 1 (PP1) complex, which associates with over 200 regulatory subunits to target hundreds of biological substrates.
• PP1 holoenzymes are essential for cell division, glycogen metabolism, muscle contractility, protein synthesis, and the regulation of synaptic plasticity.
• As a core component of the SHOC2-MRAS-PP1c (SMP) holophosphatase complex, PP1 beta specifically dephosphorylates RAF1 on Ser-259, BRAF on Ser-365, and ARAF on Ser-214, thereby stimulating their kinase activities in the MAPK signaling cascade. Related references: PMID:35768504, PMID:35831509, PMID:36175670
• In balance with CSNK1D and CSNK1E, PP1 beta regulates the circadian period length by controlling the speed and rhythmicity of PER1 and PER2 phosphorylation.
• In regulatory T cells from rheumatoid arthritis patients, PP1 beta dephosphorylates FOXP3 on Ser-418, leading to FOXP3 inactivation and Treg dysfunction. Related references: PMID:23396208
• Subcellular localization spans the cytoplasm, nucleus, nucleoplasm, and nucleolus.
• The protein undergoes acetylation and phosphorylation, requires manganese as a metal cofactor, and is linked to biological rhythms, carbohydrate metabolism, and disease variants.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide from amino acids 228–327 of human PP1 beta, a region conserved in mouse and rat, supporting the observed cross-reactivity.
• For Western blotting (WB), expect a band at approximately 37 kDa; include appropriate loading controls and verify specificity in your sample system.
• In immunohistochemistry (IHC-P) and immunofluorescence (IF/ICC), antigen retrieval conditions and fixation methods may affect epitope accessibility; consider optimization for your tissue or cell type.
• For ELISA, this antibody can be used as a capture or detection reagent; pairing with a validated secondary antibody and testing against recombinant protein or known positive lysates is advisable.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio equips diagnostic manufacturers and research teams with streamlined, end-to-end access to IVD raw materials—from concept to clinic. Our PP1 beta antibody supports studies in kinase signaling, cell cycle regulation, and oncology, backed by scalable supply and technical integration services. Beyond this target, CamelBio offers a broad portfolio of validated antibody pairs, optimized monoclonals, bulk ancillary reagents, and rare-target sourcing to accelerate your assay development.