Anti-POU6F1 Rabbit Polyclonal Antibody for WB, IF/ICC, ELISA - Q14863

Core Product Specifications and Parameters

Biological Background: POU6F1 Function and Localization

• POU6F1, also known as Brain-5 (BRN5) or mPOU homeobox protein, is a POU domain transcription factor encoded by the POU6F1 gene (GeneID: 5463) on human chromosome 12.
• It belongs to the class 6 POU domain family, characterized by a bipartite DNA-binding domain consisting of a POU-specific domain and a POU homeodomain.
• POU6F1 functions as a transcription regulator by preferentially binding to a variant octamer motif (5′-ATGATAAT-3′) to control target gene expression.
• The protein is exclusively localized to the nucleus, consistent with its role in transcriptional regulation and DNA binding.
• During embryonic development, POU6F1 expression is restricted to the developing brain, while in adult tissues, it is detected in the brain, heart, skeletal muscle, and lung, with highest levels in specific cortical layers, olfactory bulb, hippocampus, and cerebellum.
• The POU6F1 protein is a reference proteome member with documented 3D structures, and its activity may be modulated by post-translational modifications, as indicated by proteomics identification keywords.

Experimental Guidance and Technical Tips

• This rabbit polyclonal antibody was raised against an N-terminal recombinant fragment (aa 1-301) of human POU6F1, and may recognize epitopes across the full-length protein.
• For Western blotting, consider using positive control lysates from brain, heart, or skeletal muscle tissues where POU6F1 is reported to be expressed; a band around 33kDa may be observed, though the predicted full-length molecular weight is larger.
• In immunofluorescence/immunocytochemistry (IF/ICC), nuclear localization is expected; include appropriate nuclear counterstains and controls to confirm specificity.
• For ELISA, this antibody can be used as a capture or detection reagent; optimization of coating concentrations and detection conditions is recommended.
• The antibody is reported to cross-react with mouse and rat POU6F1, making it suitable for studies in these species; however, validation in the specific sample system is advised.
• Always pre-test in your own experimental system to determine optimal working dilutions and conditions, as performance may vary.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive sourcing partner for IVD raw materials, offering not only this anti-POU6F1 polyclonal antibody but also validated antibody pairs, monoclonal alternatives, and bulk ancillary reagents to support transcription factor research and neuroscience assay development. Our platform streamlines procurement from discovery to clinical application, facilitating access to rare target reagents and technical consulting.