Anti-POU4F3 Polyclonal Antibody for WB, ELISA - Q15319

Core Product Specifications and Parameters

Biological Background: POU4F3 Function and Localization

• POU4F3 (also known as BRN3C, Brain-3C) is a POU domain, class 4 transcription factor 3 that belongs to the POU family of transcription factors.
• It functions as a transcriptional activator by binding to the consensus octamer motif 5'-ATGCAAAT-3' in the regulatory regions of target genes. Related references: PMID:18228599
• POU4F3 is essential for auditory system development, playing a critical role in the terminal differentiation of hair cells in the inner ear.
• The protein localizes to both the nucleus and the cytoplasm, suggesting dynamic shuttling or context-dependent subcellular distribution.
• Tissue expression is primarily restricted to the brain, with specific enrichment in the retina.
• Keywords associated with POU4F3 include activator, DNA-binding, homeobox, deafness, and disease variant, reflecting its role in transcriptional regulation and association with non-syndromic deafness.

Experimental Guidance and Technical Tips

• The immunogen maps to the N-terminal region (amino acids 1-180) of human POU4F3. Consider whether this region is conserved in your target species orthologs if cross-reactivity is desired.
• For Western blotting, the predicted molecular weight is approximately 37 kDa. Use appropriate molecular weight markers and verify band specificity with relevant controls.
• For ELISA applications, optimize coating concentrations and antibody dilutions empirically using known positive and negative samples.
• Validate the antibody’s performance in your specific sample matrix, as expression levels may vary across tissues and cell types.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your dedicated partner for IVD raw-material sourcing, offering seamless access to validated antibodies, recombinant proteins, and ancillary reagents. For POU4F3-related research in auditory development and hearing disorders, we provide one-stop procurement support including optimized antibody pairs, bulk monoclonal/polyclonal antibodies, and rare-target raw-material sourcing. Let CamelBio bridge the gap from discovery to diagnostic application with reliable supply solutions.