Anti-POU4F2 Rabbit Polyclonal Antibody for WB, IF/ICC, ELISA - Q12837

Core Product Specifications and Parameters

Biological Background: POU4F2/BRN3B Function and Localization

• POU4F2 (BRN3B) is a tissue-specific DNA-binding transcription factor that functions as both an activator and repressor of RNA polymerase II-dependent transcription, depending on the promoter context. It is involved in the development and differentiation of target cells. Related references: PMID:19266028 PMID:23805044
• Plays a fundamental role in retinal ganglion cell (RGC) differentiation by binding to octamer motifs and forming a ternary complex with ISL1 and ISL2 to activate RGC-specific genes; it also inhibits DLX1 and DLX2 to prevent amacrine cell specification.
• Interacts with multiple transcription factors: cooperates with TP53 to promote apoptosis of neuronal cells by activating BAX, acts as a coactivator for ESR1 on estrogen-responsive promoters, and antagonizes POU4F1’s transcriptional activity.
• Primarily localizes to the nucleus, but can also be found in nuclear speckles and the cytoplasm.
• Expressed in the brain and in the ganglion cell layer of the retina. Related references: PMID:7691107
• Associated with apoptosis, alternative splicing, and developmental processes as indicated by UniProt keywords.

Experimental Guidance and Technical Tips

• The immunogen covers the N‑terminal 100 amino acids, suggesting the antibody may recognize full‑length POU4F2 and possibly alternative isoforms.
• For Western blot, a band around 43 kDa is expected; validate the band size in your specific sample type.
• In immunofluorescence/ICC, confirm nuclear localization in RGC‑related or neuronal cell lines.
• Cross‑reactivity with mouse is reported; consider verifying performance in mouse tissues or cells before use.
• For ELISA, titrate the antibody to determine optimal working concentration.

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CamelBio serves as a comprehensive IVD raw‑material sourcing partner, connecting diagnostic manufacturers and research laboratories with premium reagents like this anti‑POU4F2 polyclonal antibody. Supporting neuroscience and developmental biology research, we provide validated antibody pairs, optimized monoclonal and polyclonal antibodies, and bulk ancillary reagents to accelerate your assay development from concept to clinic.