Anti-POU4F1 Rabbit Polyclonal Antibody for WB, IF/ICC, ELISA - Q01851

Core Product Specifications and Parameters

Biological Background: POU4F1 Function and Localization

• POU4F1 (BRN3A) is a multifunctional transcription factor that binds via its C-terminal domain to the consensus octamer motif 5'-ATGCAAAT-3' in target gene regulatory regions.
• It regulates genes critical for differentiation and survival in specific neuronal lineages, with major developmental roles in somatosensory neurons and brainstem motor nuclei.
• Through its N-terminal domain, POU4F1 activates BCL2 expression to protect neuronal cells from apoptosis and induces neurite outgrowth and synaptic protein expression.
• The protein modulates estrogen receptor ESR1 transcriptional activity by enhancing its DNA-binding affinity to estrogen response elements.
• Alternative splicing generates isoforms with distinct functions; the canonical isoform 1 mediates full transcriptional and antiapoptotic effects, whereas other isoforms (e.g., isoform 2) exhibit restricted regulatory capacity.
• Subcellular localization is primarily nuclear, consistent with its role in transcription regulation, but the protein may also reside in the cytoplasm.
• POU4F1 is expressed in the brain, retina, and during development in the brain, spinal cord, and eye, underscoring its importance in neural development.
• Annotated keywords include DNA-binding, homeobox domain, developmental protein, and disease variant (associated with intellectual disability), highlighting its essential roles in gene regulation and neurodevelopment.

Experimental Guidance and Technical Tips

• For Western blot detection, expect a specific band at approximately 43 kDa using lysates from human, mouse, or rat neuronal tissues or cell lines; include appropriate positive and negative controls to confirm specificity.
• In immunofluorescence/ICC, POU4F1 localizes to both nucleus and cytoplasm; ensure proper permeabilization and fixation protocols optimized for nuclear antigens.
• The immunogen targets the N-terminal region (aa 1-100); isoforms lacking this domain may not be recognized, so consider this when interpreting results for different splice variants.
• For ELISA applications, the polyclonal nature provides robust capture/detection capabilities; optimize coating buffers and blocking conditions for your specific assay format.
• Cross-reactivity with mouse and rat is reported, but validation in the exact sample system is recommended before large-scale use.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your dedicated partner for IVD raw-material procurement, offering this anti-POU4F1 antibody along with a comprehensive portfolio of validated antibodies, bulk ancillary reagents, and customized solutions. Whether you require antibody pairs, rare target sourcing, or technical support from concept to clinic, our platform bridges gaps in neuroscience and transcription factor research. Count on us to deliver reliable, scalable materials that accelerate your diagnostic development pipeline.