Anti-POU3F4 Polyclonal Antibody for WB and ELISA - P49335

Core Product Specifications and Parameters

Biological Background: POU3F4 Function and Localization

• POU3F4, also known as Brain-4 (BRN-4) or Oct-9, is a member of the POU domain class 3 transcription factor family.
• It functions as a probable transcription factor, playing a key role during early neural development and in a limited set of neurons in the mature brain.
• The protein contains a POU domain, a bipartite DNA-binding domain, and a homeobox domain, characteristic of this transcription factor family.
• POU3F4 is expressed specifically in the brain.
• It localizes to the nucleus, consistent with its role in transcriptional regulation.
• Mutations in POU3F4 are associated with non-syndromic deafness (DFNX2), highlighting its role in auditory system development.
• The protein is subject to phosphorylation, as indicated by its inclusion in proteomics identification studies.
• Keywords associated with POU3F4 include deafness, disease variant, DNA-binding, homeobox, nucleus, phosphoprotein, and transcription regulation.

Experimental Guidance and Technical Tips

• The immunogen is a recombinant fusion protein covering the N-terminal region (a.a. 1-190) of human POU3F4; this region includes part of the POU domain and may contribute to antibody specificity.
• For Western blot (WB), the antibody detects a band at approximately 39 kDa; consider using nuclear extracts for optimal detection due to POU3F4’s nuclear localization.
• ELISA applications may be optimized using recombinant POU3F4 protein or peptide antigens; validate cross-reactivity with mouse and rat samples if endogenous detection is required.
• When working with tissue samples, prioritize brain tissue lysates, as POU3F4 is brain-specific; consider enrichment of nuclear fractions to enhance signal.
• Validate antibody specificity in your experimental system using appropriate controls, such as knockdown or knockout cell lines, or peptide blocking.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, labs, and research institutes with one-stop access to IVD raw materials, technical services, and consulting. For POU3F4 and other neural transcription factor targets, we offer a range of validated antibody products, including polyclonal antibodies like this one, alongside optimized antibody pairs and bulk ancillary reagents. Our procurement capabilities also extend to rare target raw-material sourcing, supporting your workflow from concept to clinic.