Anti-POU3F2 Polyclonal Antibody for WB, ELISA - P20265

Core Product Specifications and Parameters

Biological Background: POU3F2 Function and Localization

• POU3F2 (also known as BRN2, OCT7, OTF-7, POUF3, N-Oct3) is a member of the POU domain class 3 transcription factor family, encoded by the POU3F2 gene.
• It binds to the consensus DNA sequence consisting of half-sites ('GCAT') and ('TAAT') separated by a variable spacer, regulating transcription of neuronal genes.
• Acts as a transcriptional activator in synergy with SOX4, SOX11, or SOX12 to promote gene expression.
• Together with ASCL1 and MYT1L, POU3F2 can reprogram fibroblasts into induced neuronal (iN) cells, acting downstream of ASCL1 to access open chromatin.
• Localized exclusively in the nucleus, consistent with its role as a transcription factor.
• The protein is subject to phosphorylation (as indicated by the keyword 'Phosphoprotein'), which may modulate its activity.
• Expression is restricted to the neuroectodermal lineage, underscoring its role in neuronal differentiation and neurogenesis.
• The theoretical molecular weight is approximately 46.9 kDa (UniProt: P20265), with observed migration at approximately 47 kDa by SDS-PAGE.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the N-terminal region (aa 1-68) of human POU3F2; validate reactivity in your target species and sample type.
• For Western blot, consider using whole-cell lysates from neuronal cell lines (e.g., SH-SY5Y) or brain tissue extracts. The predicted band is ~47 kDa; confirm with a positive control.
• For ELISA, titrate the antibody against the immunogen peptide or recombinant protein to determine optimal working dilution.
• As a polyclonal antibody, it may recognize multiple isoforms or degradation products; include appropriate controls.
• Cross-reactivity has been reported for human; if working with other species, verify sequence homology of the immunogen.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers a comprehensive sourcing platform for IVD raw materials, including this POU3F2 polyclonal antibody validated for WB and ELISA. In addition to antibodies targeting key transcriptional regulators in neurobiology, we supply optimized antibody pairs, bulk ancillary reagents, and rare-target raw materials to support your diagnostic development from concept to clinic.