Anti-POU3F1 Polyclonal Antibody for WB, ELISA - Q03052

Core Product Specifications and Parameters

Biological Background: POU3F1 Function and Localization

• POU3F1 (also known as Oct-6, OTF-6, SCIP) is a transcription factor belonging to the POU domain class 3 family. It binds specifically to the octamer motif 5'-ATTTGCAT-3'.
• Functions as both a transcriptional activator and repressor. It activates transcription when binding cooperatively with SOX4, SOX11, or SOX12, and represses myelin-specific gene expression.
• Localizes primarily to the nucleus, consistent with its role in DNA binding and transcription regulation.
• Expressed in embryonal stem cells and the developing brain, suggesting roles in early development and neural differentiation.
• Contains a POU-specific domain and a homeobox domain, characteristic of POU family transcription factors.
• The protein has a molecular weight of approximately 45 kDa.

Experimental Guidance and Technical Tips

• The immunogen used is full-length human POU3F1 (amino acids 1–260), which may recognize endogenous POU3F1 across human, mouse, and rat due to high sequence homology (cross-reactivity confirmed for mouse and rat).
• For Western blot, a band at 45 kDa is expected. Consider using nuclear extracts or whole cell lysates from relevant cell lines (e.g., HeLa, neuroblastoma) and validate antibody specificity with knockdown or knockout controls.
• For ELISA, the antibody can be used as a capture or detection antibody; titrate to determine optimal working concentration for your assay system.
• The polyclonal nature may provide robust signal but consider batch-to-batch variability; validate each new lot in your application.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers one-stop access to a broad portfolio of IVD raw materials, including validated antibody pairs, optimized monoclonal/polyclonal antibodies, and bulk ancillary reagents. As a sourcing partner, we connect diagnostic manufacturers and research labs with reliable, high-quality reagents like this anti-POU3F1 antibody to support your transcription factor research and assay development. From concept to clinic, CamelBio streamlines your raw-material procurement, enabling you to focus on innovation.