Anti-POU2F3 Rabbit Polyclonal Antibody for WB, ELISA - Q9UKI9

Core Product Specifications and Parameters

Biological Background: POU2F3 Function and Localization

• POU2F3, also known as Oct-11, OTF-11, PLA-1, or Skn-1, is a POU domain class 2 transcription factor encoded by the POU2F3 gene (alternative splicing produces isoforms).
• It binds the octamer motif (5′-ATTTGCAT-3′) and regulates cell type-specific differentiation pathways, including keratinocyte differentiation. Related references: PMID:11329378
• The POU2F3-POU2AF2/POU2AF3 complex drives the expression of tuft-cell-specific genes, coordinating immune and neural functions in mucosal epithelia. Related references: PMID:35576971
• POU2F3 localizes to the nucleus, consistent with its role as a transcription factor.
• It is specifically expressed in the epidermis and cultured keratinocytes, indicating a tissue-restricted regulatory function.
• Contains POU-specific and homeobox DNA-binding domains, classified under keywords: DNA-binding, homeobox, transcription factor; also subject to alternative splicing.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the N-terminal region (aa 1–180) of human POU2F3, which may aid in detecting specific isoforms; confirm specificity in your experimental system.
• For WB analysis, consider using extracts from human keratinocytes or epidermal tissues, where POU2F3 is specifically expressed.
• Reported cross-reactivity with mouse suggests potential utility in mouse skin or epithelial models; validate in the relevant sample system.
• ELISA can be employed for quantitation of POU2F3 levels in cellular or tissue lysates, with appropriate standard curves.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, labs, and research institutes with one-stop access to IVD raw materials, technical services, and consulting—covering every stage from concept to clinic. This anti-POU2F3 polyclonal antibody supports research into keratinocyte biology and mucosal immunity. As your sourcing partner, we offer validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and rare target raw-material sourcing to accelerate your diagnostic and research workflows.