Anti-POU2F1/OCT1 Rabbit Monoclonal Antibody for WB, IHC-P, ELISA - P14859

Core Product Specifications and Parameters

Biological Background: POU2F1/OCT1 Function and Localization

• POU2F1 (also known as Oct-1, OTF-1, NF-A1) is a ubiquitously expressed transcription factor, with a lymphocyte-specific isoform (isoform 2) generated by alternative splicing.
• It binds to the octamer motif (5'-ATTTGCAT-3') to activate promoters of small nuclear RNA (snRNA) genes, histone H2B, and immunoglobulin genes.
• POU2F1 modulates transcriptional transactivation by nuclear receptors such as NR3C1, androgen receptor (AR), and progesterone receptor (PGR).
• During herpes simplex virus (HSV) infection, POU2F1 forms a multiprotein-DNA complex with viral transactivator VP16 and HCFC1, enabling transcription of viral immediate early genes.
• The protein localizes to the nucleus and contains both a POU-specific domain and a homeobox domain for DNA binding.
• Post-translational modifications include phosphorylation, as indicated by the Phosphoprotein keyword.
• It is classified under functional keywords: Activator, Alternative splicing, DNA-binding, Homeobox, Nucleus, Phosphoprotein, Reference proteome, Transcription, and Transcription regulation.

Experimental Guidance and Technical Tips

• For Western blot, target a band at approximately 76 kDa; validate detection across a panel of cell lines or tissues due to its ubiquitous expression. The lymphocyte-specific isoform may require confirmation in lymphoid samples.
• In IHC-P, optimize antigen retrieval and antibody dilution for the tissue of interest, and include appropriate controls to discriminate specific nuclear staining.
• As the immunogen is recombinant full-length or partial protein (not specified), cross-reactivity between isoforms is possible; verify specificity by comparing lymphocyte versus non-lymphocyte lysates.
• For ELISA, consider direct coating with recombinant POU2F1 antigen or use a capture-detection antibody pair format; assess linearity and sensitivity with known standards.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive IVD raw-material partner for diagnostic manufacturers, labs, and research institutes, covering every step from concept to clinic. For transcription factor and viral infection research, we provide validated antibody pairs, optimized monoclonal antibodies like this POU2F1/OCT1 clone, and bulk ancillary reagents. Our sourcing expertise extends to rare or custom targets, streamlining your procurement and enabling robust assay development.