Anti-POU2AF1 Polyclonal Antibody for WB and ELISA - Q16633

Core Product Specifications and Parameters

Biological Background: POU2AF1 Function and Localization

• POU domain class 2-associating factor 1 (POU2AF1), also known as BOB1, OBF1, or OCA-B, functions as a transcriptional coactivator.
• It specifically associates with the POU domains of transcription factors POU2F1/OCT1 and POU2F2/OCT2, enhancing their promoter activity (PubMed:7859290, PubMed:7779176).
  Related references: PMID:7859290 PMID:7779176
• POU2AF1 is essential for B-cell response to antigens and is required for the formation of germinal centers (PubMed:7623806, PubMed:7859290).
  Related references: PMID:7623806 PMID:7859290
• It also regulates IL6 expression in B cells as a coactivator of POU2F2/OCT2 (By similarity).
• The protein localizes exclusively to the nucleus, consistent with its role in transcription regulation.
• POU2AF1 is predominantly expressed in B cells and detected in spleen, thymus, peripheral blood leukocytes, and small intestine (PubMed:7779176, PubMed:7859290).
  Related references: PMID:7779176 PMID:7859290
• As a proto-oncogene, POU2AF1 is subject to chromosomal rearrangements and post-translational modifications such as ubiquitination and sumoylation, which may modulate its activity.

Experimental Guidance and Technical Tips

• The immunogen comprises amino acids 1-256 of human POU2AF1 (NP_006226.2), covering the N-terminal region. Consider performing epitope mapping if precise binding domain information is critical.
• This antibody is recommended for Western blot (WB) and ELISA applications. For WB, the predicted molecular weight of POU2AF1 is approximately 27 kDa; validate the band size in your sample system.
• Confirmed cross-reactivity with human, mouse, and rat POU2AF1. When using in mouse or rat models, validate reactivity in the relevant tissue or cell lysate (e.g., spleen, B-cell lines).
• Optimize antibody dilution and incubation conditions for each application and sample type, as performance may vary.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers diagnostic manufacturers, laboratories, and research institutes a one-stop sourcing platform for IVD raw materials, including validated antibody pairs, optimized monoclonal/polyclonal antibodies, and bulk ancillary reagents. Whether you need high-quality POU2AF1 antibodies for B-cell research, immunoassay development, or rare-target raw-material sourcing, our team supports your projects from concept to clinic.