Anti-POU1F1 Rabbit Polyclonal Antibody for WB, IF-P, IHC-P, ELISA - P28069

Core Product Specifications and Parameters

Biological Background: POU1F1 Function and Localization

• POU1F1, also known as PIT1 or GHF-1, is a pituitary-specific transcription factor essential for anterior pituitary development and hormone regulation.
• It specifically binds to the consensus DNA sequence 5'-TAAAT-3' to activate transcription of growth hormone and prolactin genes (PubMed:22010633, PubMed:26612202). Related references: PMID:22010633, PMID:26612202
• POU1F1 is a member of the POU domain transcription factor family and contains a homeobox domain.
• It localizes to the nucleus, where it regulates gene expression.
• Mutations in POU1F1 are associated with combined pituitary hormone deficiency (CPHD1) and dwarfism, highlighting its critical role in endocrine function.
• Alternative splicing generates isoforms; the protein has a molecular weight of approximately 33 kDa.
• Key post-translational modifications and structural details have been studied, contributing to understanding its DNA-binding and transactivation mechanisms.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the N-terminal region (aa 1-150) of human POU1F1; therefore, the antibody may detect all isoforms if the epitope is conserved.
• For Western blot, expect a band at approximately 33 kDa; validate in pituitary-derived cell lines or tissues known to express POU1F1.
• In immunofluorescence or immunohistochemistry, anticipate nuclear staining; optimize antigen retrieval and antibody dilution in the relevant sample system.
• Consider cross-adsorption if using in multi-species experiments beyond human, mouse, and rat.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your one-stop partner for IVD raw materials, offering integrated supply solutions from concept to clinic. For pituitary and endocrine research, we provide optimized polyclonal and monoclonal antibodies, validated antibody pairs, and bulk ancillary reagents to support your diagnostic development. Our sourcing expertise ensures reliable access to rare target raw materials like POU1F1 antibodies, enabling streamlined assay development.