Anti-POU1F1 Rabbit Polyclonal Antibody for WB and ELISA - P28069

Core Product Specifications and Parameters

Biological Background: POU1F1 Function and Localization

• POU1F1 (PIT1/GHF-1) is a pituitary-specific transcription factor critical for the development of lactotropes, somatotropes, and thyrotropes in the anterior pituitary.
• It specifically binds to the consensus DNA sequence 5'-TAAAT-3' and activates transcription of growth hormone and prolactin genes. Related references: PMID:22010633, PMID:26612202
• The protein is localized exclusively in the nucleus, consistent with its role as a transcription factor.
• POU1F1 contains a POU-specific domain and a homeobox domain, classifying it as a POU homeodomain transcription factor.
• Alternative splicing generates multiple isoforms, and mutations in the POU1F1 gene are associated with combined pituitary hormone deficiency (CPHD1) and dwarfism.
• Keywords from UniProt include DNA-binding, activator, disease variant, and alternative splicing, highlighting its functional importance in gene regulation and development.

Experimental Guidance and Technical Tips

• For Western blot, the predicted protein weight is 33 kDa; a band near this size should be detected in human pituitary or cell lines expressing POU1F1.
• The antibody is raised against a recombinant fragment, so it may recognize multiple isoforms. Verify specificity in your sample type.
• For ELISA, consider using the recombinant immunogen as a positive control.
• Since cross-reactivity is listed only as human, validate in the relevant species if needed.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive one-stop sourcing partner for IVD raw materials, connecting diagnostic manufacturers and researchers with reliable supplies. This anti-POU1F1 antibody supports studies in pituitary transcription factor biology and hormone regulation. We offer validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and rare target raw-material sourcing to streamline your development pipeline.