Anti-POT1 Rabbit Polyclonal Antibody for WB, ELISA - Q9NUX5

Core Product Specifications and Parameters

Biological Background: POT1 Function and Localization

• POT1 (Protection of telomeres protein 1) is a component of the shelterin complex (telosome) that associates with double‑stranded TTAGGG telomeric repeats to protect chromosome ends from inappropriate DNA repair.
• It is also part of the telomerase ribonucleoprotein complex and contributes to telomere length regulation through cis‑inhibition of telomerase and as a downstream effector of the TRF1 complex.
• The protein binds specifically to single‑stranded telomeric G‑strand repeats (5'‑TTAGGG‑3') with high affinity for the minimal 5'‑TAGGGTTAG‑3' sequence, acting near or at 3' ends.
• POT1 forms a heterodimer with ACD that enhances telomere elongation by increasing telomerase processivity, a TERT‑dependent activity.
• Subcellular localization is restricted to the nucleus, specifically at chromosome telomeres.
• The gene is ubiquitously expressed and undergoes alternative splicing, and mutations are associated with several disease phenotypes (CMM10, GLM9).

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 497–634 of human POT1, a region that may provide specific recognition of the protein in western blot and ELISA.
• For WB, consider using whole‑cell lysates from human or mouse cell lines; validate specificity with appropriate positive and negative controls.
• In ELISA, the antibody can be used as a capture or detection reagent; optimal working concentrations should be titrated in the relevant sample matrix.
• Because of POT1’s role in telomere maintenance, note that expression may vary with cell proliferation state; confirm loading consistency with a housekeeping protein.

CamelBio: Your One‑Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research labs with ready access to high‑quality IVD raw materials, including validated antibodies for telomere biology targets like POT1. Our portfolio covers monoclonal and polyclonal formats, bulk ancillary reagents, and rare‑target sourcing—designed to support your assay development from concept to clinic.