Anti-PORCN Rabbit Polyclonal Antibody for WB and ELISA - Q9H237

Core Product Specifications and Parameters

Biological Background: PORCN Function and Localization

• PORCN, also known as Protein-serine O-palmitoleoyltransferase porcupine or Protein MG61, is encoded by the PORCN gene (also designated MG61, PORC, PPN) in humans.
• PORCN serves as a key regulator of the canonical Wnt signaling pathway by catalyzing the attachment of palmitoleate (a 16-carbon monounsaturated fatty acid) to Wnt proteins, which is essential for their secretion and receptor binding.
• As an acyltransferase, PORCN belongs to the membrane-bound O-acyltransferase family and contains multiple transmembrane helices, anchoring it to organelle membranes.
• PORCN localizes specifically to the endoplasmic reticulum membrane, where it modifies Wnt ligands prior to their trafficking and release.
• Multiple PORCN isoforms exist due to alternative splicing, with isoform 1 prominently expressed in fetal and adult brain regions, heart, skeletal muscle, and testis, while isoform 4 is detected in a different set of tissues including kidney, liver, lung, and spleen.
• Mutations in PORCN are associated with focal dermal hypoplasia (Goltz syndrome), highlighting its critical role in development and linking it to the disease variant keyword.

Experimental Guidance and Technical Tips

• Given the immunogen corresponds to amino acids 220-320 of human PORCN, this antibody may recognize a sequence within the C-terminal region of the protein; for western blotting, consider using reducing conditions and a positive control lysate from tissues with high PORCN expression such as testis or brain.
• For ELISA applications, we recommend optimizing coating antigen concentration and antibody dilution in a checkerboard titration, and validating specificity using recombinant PORCN protein or peptide blocking.
• Since PORCN is a low-abundance transmembrane protein, enrichment of membrane fractions or immunoprecipitation prior to WB may improve detection sensitivity.
• Cross-reactivity with mouse and rat PORCN has been indicated; however, we advise confirming reactivity in your specific experimental system due to potential epitope variability.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio empowers diagnostic manufacturers and research institutions by providing a comprehensive portfolio of IVD raw materials, including validated antibody pairs, optimized monoclonal and polyclonal antibodies, and specialized reagents for Wnt signaling and beyond. This anti-PORCN polyclonal antibody exemplifies our commitment to supporting critical pathway analysis and assay development. As a one-stop sourcing bridge, we streamline the procurement of rare-target raw materials and ancillary reagents, enabling seamless transition from concept to clinical application.