Anti-POP7 Rabbit Polyclonal Antibody for WB, ELISA - O75817

Core Product Specifications and Parameters

Biological Background: POP7 Function and Localization

• POP7 encodes the ribonuclease P protein subunit p20 (also known as RPP20/hPOP7), a core component of the RNase P ribonucleoprotein complex responsible for generating mature tRNA molecules by cleaving their 5′-ends. Related references: PMID:30454648, PMID:9630247
• POP7 is also a subunit of the MRP ribonuclease complex, which processes pre-ribosomal RNA (pre-rRNA) sequences. Related references: PMID:28115465
• The protein is characterized by an RNA-binding domain and belongs to the ribonuclease P/MRP protein family.
• Subcellularly, POP7 localizes predominantly to the nucleolus and nucleus, with additional presence in the cytoplasm, particularly within cytoplasmic granules.
• Functional studies indicate its essential role in tRNA and rRNA maturation pathways, linking POP7 to fundamental cellular housekeeping processes.

Experimental Guidance and Technical Tips

• The immunogen spans nearly the full-length POP7 protein (aa 1–140 of 140), raising the likelihood of detecting native conformations; verify detection of endogenous POP7 in your target sample system.
• For Western blot, a band around 16 kDa is expected; consider using appropriate positive (e.g., HeLa or HEK293 whole-cell lysates) and negative controls.
• ELISA applications may benefit from coating the recombinant immunogen as a positive control; optimize antibody dilution for your specific assay format.
• Due to its nucleolar localization, cell fractionation may enrich the signal in nuclear/nucleolar extracts.
• Cross-reactivity is reported for human only; confirm reactivity in other species if needed.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive sourcing bridge for IVD manufacturers and research laboratories, offering validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and customized raw-material solutions. This anti-POP7 polyclonal antibody exemplifies our portfolio targeting nucleic acid metabolism pathways, with applications in tRNA and rRNA processing research. Contact our team to explore full supply chain capabilities for your diagnostic development needs.