Anti-POP5 Rabbit Polyclonal Antibody for WB and ELISA - Q969H6

Core Product Specifications and Parameters

Biological Background: POP5 Function and Localization

• POP5 (also known as RPP2, RPP20, hPop5, or HSPC004) is a protein subunit of the ribonuclease P and MRP complexes.
• It is an essential component of ribonuclease P, which catalyzes the cleavage of the 5′ leader sequences from precursor tRNAs to generate mature tRNA molecules (PubMed:11413139, PubMed:30454648). Related references: PMID:11413139 PMID:30454648
• POP5 also participates in the MRP ribonuclease complex, which processes precursor ribosomal RNA (PubMed:28115465). Related references: PMID:28115465
• The protein localizes predominantly to the nucleolus within the nucleus.
• POP5 is alternatively spliced and has been identified as a phosphoprotein, suggesting post‑translational regulation.
• Experimental evidence supports its RNA‑binding function, consistent with its role in ribonucleoprotein complexes.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide spanning amino acids 50–150; consider that the antibody may not recognize epitopes if denatured or masked in certain conformations.
• For Western blot, endogenous POP5 is expected at ~19 kDa; validate detection in your specific sample system, and account for possible post‑translational modifications that may shift the band.
• For ELISA, standard indirect or sandwich formats can be attempted; note that the antibody cross‑reactivity with human and rat indicates potential use in rat‑derived samples.
• Always include appropriate positive and negative controls to confirm specificity and minimize background.

CamelBio: Your One-Stop Sourcing Bridge

Leverage CamelBio’s end‑to‑end supply capabilities for your IVD raw material needs. This anti‑POP5 antibody is part of a broader portfolio supporting research in ribonuclease biology and RNA processing. CamelBio offers validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and specialized sourcing for rare targets—helping diagnostic manufacturers and labs accelerate development from concept to clinic.