Anti-POP4 Rabbit Polyclonal Antibody for WB, ELISA - O95707

Core Product Specifications and Parameters

Biological Background: POP4 Function and Localization

• POP4 (Processing of Precursor 4), also known as RPP29, encodes the ribonuclease P protein subunit p29, a key component of the nuclear RNase P complex.
• RNase P is a ribonucleoprotein that catalyzes the cleavage of the 5'-leader sequence from precursor tRNA molecules, an essential step in tRNA maturation.
• The protein localizes to the nucleolus within the nucleus, consistent with its role in ribonucleoprotein assembly and tRNA processing.
• POP4 is classified as an RNA-binding protein and phosphoprotein, with post-translational phosphorylation potentially regulating its activity.
• The protein has a calculated molecular mass of approximately 25.4 kDa (25425 Da), matching the experimentally observed weight of 25 kDa.
• Structural studies have resolved the 3D conformation of POP4, supporting its functional characterization.
• As a reference proteome member identified by direct protein sequencing, POP4 is a conserved and well-characterized factor in tRNA biogenesis.

Experimental Guidance and Technical Tips

• The immunogen encompasses full-length human POP4 (residues 1-220); therefore, the antibody may recognize both native and denatured conformations, making it suitable for applications such as western blotting and ELISA.
• For WB analysis, prepare nuclear or whole-cell lysates and detect a band around 25 kDa. Validate specificity using a knockdown or knockout control, if available.
• For ELISA, consider using the antibody as a capture or detection reagent; titrate appropriately for direct or indirect formats.
• Confirmed cross-reactivity with human and rat samples; for other species, check sequence homology of the immunogen region before use.
• Because POP4 functions in a ribonucleoprotein complex, co‑immunoprecipitation or pull‑down experiments may be explored to study protein partners.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, research labs, and institutes with consolidated access to IVD raw materials, technical support, and consulting—from early concept through clinical application. Our portfolio includes validated antibody pairs, optimized polyclonal and monoclonal antibodies, bulk ancillary reagents, and tailored sourcing for rare targets like ribonuclease P subunits. Whether you are developing tRNA processing assays or nucleolar research tools, our supply chain expertise helps you secure reliable, high-quality materials efficiently.