Anti-POP4 Polyclonal Antibody for WB, IHC-P, ELISA - O95707

Core Product Specifications and Parameters

Biological Background: POP4 Function and Localization

• POP4, also known as RPP29, is a protein subunit of ribonuclease P (RNase P), a ribonucleoprotein complex that processes precursor tRNAs.
• The primary function of POP4 is to cleave the 5'-leader sequences from pre-tRNAs, generating mature tRNA molecules essential for protein synthesis.
• POP4 localizes predominantly to the nucleolus within the nucleus, aligning with its role in ribosome biogenesis and tRNA processing.
• It contains an RNA-binding domain that facilitates its direct interaction with the RNA component of the RNase P complex.
• Phosphoproteomic studies have identified POP4 as a phosphoprotein, suggesting that phosphorylation may modulate its activity or complex assembly.
• The three-dimensional structure of POP4 has been resolved, offering detailed insights into its molecular interactions within the RNase P holoenzyme.
• As a reference proteome component, POP4 is conserved across species, underscoring its fundamental role in cellular function.

Experimental Guidance and Technical Tips

• The immunogen spans the full-length POP4 (aa 1-220), which may enable recognition of both phosphorylated and unphosphorylated forms; verify the detection of specific PTMs if relevant.
• For Western blotting, use reducing conditions and include a positive control such as a lysate from HeLa or HEK293 cells, where POP4 is expected at ~25 kDa.
• In IHC-P, antigen retrieval may be necessary; optimize using tissues with high nucleolar content (e.g., liver, testis) and validate with appropriate controls.
• Cross-reactivity with mouse and rat is reported; however, sequence alignment of the immunogen region is recommended to confirm epitope conservation.
• For ELISA, consider pairing with a capture antibody if developing a sandwich assay, and validate the detection range using recombinant POP4 protein.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers diagnostic manufacturers and researchers streamlined access to high-quality IVD raw materials, including this anti-POP4 polyclonal antibody for tRNA processing studies. Complementing your research, our portfolio features validated antibody pairs, bulk ancillary reagents, and sourcing support for rare targets to accelerate assay development from concept to clinic. For customized inquiries, contact our expert team.