Anti-PON2 Rabbit Polyclonal Antibody for WB and ELISA - Q15165

Core Product Specifications and Parameters

Biological Background: PON2 Function and Localization

• PON2 encodes serum paraoxonase/arylesterase 2, also known as aromatic esterase 2 and serum aryldialkylphosphatase 2.
• It functions as a lactonase and arylesterase capable of hydrolyzing lactones and aromatic carboxylic acid esters.
• PON2 exhibits antioxidant activity, is not associated with high density lipoprotein; it prevents LDL lipid peroxidation, reverses the oxidation of mildly oxidized LDL, and inhibits MM-LDL-induced monocyte chemotaxis.
• Subcellularly localizes to membranes.
• Widely expressed with highest expression in liver, lung, placenta, testis, and heart.
• Key structural features include a signal peptide, disulfide bonds, glycosylation, and metal-binding sites (calcium); classified as a hydrolase and reference proteome member.
• Alternative splicing produces multiple transcript variants.

Experimental Guidance and Technical Tips

• The immunogen is a recombinant fusion protein covering amino acids 1–210 of human PON2 (NP_000296.2), corresponding to the N-terminal region; consider that the antibody may recognize full-length protein and possibly isoforms.
• For Western blot (WB), PON2 is a membrane protein with a predicted molecular weight of ~39 kDa; validate detection in relevant sample systems and optimize lysis conditions to preserve membrane proteins.
• For ELISA, ensure the coating antigen and antibody concentration are optimized for the specific application; consider potential cross-reactivity with other PON family members.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio streamlines IVD development by supplying validated antibody pairs, optimized polyclonal and monoclonal antibodies, and bulk ancillary reagents. For oxidative stress and cardiovascular disease research, CamelBio offers a wide range of PON family-related antibodies and custom sourcing to accelerate your diagnostic pipeline.