Anti-PON1 Rabbit Polyclonal Antibody for WB, ELISA - P27169

Core Product Specifications and Parameters

Biological Background: PON1 Function and Localization

• PON1 (paraoxonase 1) is a calcium-dependent hydrolase that belongs to the serum paraoxonase/arylesterase family. It is also designated as aromatic esterase 1 (A-esterase 1) and K-45.
• The enzyme catalyzes the hydrolysis of toxic organophosphate metabolites from insecticides, as well as a broad range of lactones and aromatic carboxylic acid esters.
• PON1 plays a protective role against oxidative stress by preventing the oxidation of low-density lipoproteins (LDL), thus inhibiting atheroma formation.
• Secreted into the extracellular space, PON1 circulates in plasma primarily associated with high-density lipoprotein (HDL) particles.
• The protein is synthesized predominantly in the liver, with expression largely absent in other tissues like heart, brain, placenta, lung, skeletal muscle, kidney, or pancreas.
• Key structural features include disulfide bonds, glycosylation, and calcium-binding sites, which are essential for its enzymatic activity.
• The mature protein has a molecular weight of approximately 40 kDa (UniProt: 39731 Da), consistent with the value reported for this antibody.

Experimental Guidance and Technical Tips

• The immunogen covers amino acids 20–150, a conserved region important for enzymatic function; this antibody is suitable for detecting both native and denatured PON1.
• For Western blot, consider using a dilution series to optimize signal detection, given the expected band at ~40 kDa.
• The antibody cross-reacts with mouse and rat PON1, making it applicable for multi-species studies; always validate in your specific experimental system.
• ELISA applications may require optimization of coating conditions and detection antibodies; pairing with a validated standard can improve quantitation.

CamelBio: Your One-Stop Sourcing Bridge

As a dedicated IVD raw-material supplier, CamelBio offers comprehensive sourcing solutions for research and diagnostic development, including this anti-PON1 polyclonal antibody. Our portfolio includes validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and support for rare target procurement to accelerate your projects from concept to clinic. Whether you are investigating organophosphate detoxification or cardiovascular biomarkers, CamelBio can streamline your material sourcing needs.