Anti-PON1 Rabbit Monoclonal Antibody for WB, IF-P, IHC-P, ELISA - P27169

Core Product Specifications and Parameters

Biological Background: PON1 Function and Localization

• PON1 encodes serum paraoxonase/arylesterase 1, also known as aromatic esterase 1 (A-esterase 1), K-45, and serum aryldialkylphosphatase 1.
• It is a calcium-dependent hydrolase that catalyzes the breakdown of toxic organophosphate insecticide metabolites, lactones, and aromatic carboxylic acid esters.
• PON1 protects low-density lipoproteins (LDL) from oxidative modification, contributing to anti-atherogenic mechanisms in the bloodstream.
• The protein is secreted and found extracellularly, predominantly associated with high-density lipoprotein (HDL) particles in plasma.
• Expression is highly tissue-specific: detected in liver at the protein level, but absent from heart, brain, placenta, lung, skeletal muscle, kidney, and pancreas.
• Post-translational features include a signal peptide, disulfide bonds, glycosylation, and a calcium-binding site, all consistent with its stable secretion and enzymatic function.

Experimental Guidance and Technical Tips

• The immunogen lies within residues 50–150 of human PON1, targeting an N-terminal region; consider this when designing epitope-mapping or competitive assays.
• For Western blotting, a band near 40 kDa is expected under reducing conditions; note that the theoretical molecular weight from UniProt is approximately 39.7 kDa.
• In immunohistochemistry (IHC-P), antigen retrieval may be necessary, and liver tissue sections can serve as a positive control given the strong hepatic expression.
• When using immunofluorescence (IF-P), anticipate a secretory/extracellular staining pattern; co-localization with HDL markers can confirm specificity.
• Cross-reactivity with mouse and rat is reported; validate in the appropriate species and tissue context before quantitative studies.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supports diagnostic manufacturers and research labs with one-stop access to IVD raw materials like this anti-PON1 monoclonal antibody, ideal for studying organophosphate detoxification and HDL antioxidant function. We provide validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and sourcing for rare targets to streamline your assay development from concept to clinic.