Anti-POMT1 Rabbit Polyclonal Antibody for WB, ELISA - Q9Y6A1

Core Product Specifications and Parameters

Biological Background: POMT1 Function and Localization

• POMT1 (Protein O-mannosyl-transferase 1) is an endoplasmic reticulum membrane protein that transfers mannosyl residues to serine or threonine residues of target proteins. Related references: PMID:12369018 PMID:14699049 PMID:28512129
• Enzymatic activity requires coexpression with POMT2; neither POMT1 nor POMT2 alone is sufficient. Related references: PMID:12369018 PMID:14699049 PMID:28512129
• POMT1 is essentially dedicated to O-mannosylation of alpha-dystroglycan (DAG1) and few other proteins, but not cadherins or protocadherins. Related references: PMID:28512129
• It is widely expressed, with highest levels in testis, heart, and pancreas, and lower expression in kidney, skeletal muscle, brain, placenta, lung, and liver.
• POMT1 contains multiple transmembrane helices and a conserved MIR (mannosyltransferase, IP3R and RyR) domain, and belongs to the glycosyltransferase family 39.
• Mutations in POMT1 are associated with congenital muscular dystrophy-dystroglycanopathy with brain and eye anomalies (types A1, B1, C1), limb-girdle muscular dystrophy type 2K (LGMD2K), and lissencephaly.
• The protein undergoes alternative splicing, producing isoforms that may have distinct functional or regulatory roles.
• Post-translational modifications include N-glycosylation and metal binding, which are critical for its transferase activity.

Experimental Guidance and Technical Tips

• For Western blot, consider using whole-cell lysates from testis or heart tissues where POMT1 expression is abundant. The predicted molecular weight is ~85kDa, but glycosylation or isoforms may cause slight size shifts.
• When setting up an ELISA, this polyclonal antibody can be titrated to serve as a coating or detection reagent. Include proper controls and confirm optimal dilutions for your specific assay format.
• As with any polyclonal antibody, lot-to-lot consistency should be verified. Validate performance in your own experimental system and consider cross-adsorption if cross-reactivity is observed in complex samples.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is a dedicated one-stop sourcing partner for IVD raw materials, offering validated antibody pairs, bulk polyclonal antibodies, and ancillary reagents to support your diagnostic or research programs. This anti-POMT1 antibody serves as a reliable tool for investigations into O-mannosylation, dystroglycanopathy, and muscular dystrophy. For larger quantities, custom conjugations, or technical consultation, please reach out to our specialist team—we bridge the gap from concept to clinic with comprehensive supply solutions.