Anti-POMP Rabbit Polyclonal Antibody for WB, ELISA - Q9Y244

Core Product Specifications and Parameters

Biological Background: POMP Function and Localization

• POMP (Proteasome maturation protein), also known as proteassemblin, hUMP1, or voltage-gated K channel beta subunit 4.1, is a molecular chaperone essential for the assembly of both standard proteasomes and immunoproteasomes.
• It mediates the association of the 20S preproteasome with the endoplasmic reticulum, facilitating the correct maturation of the proteolytic core complex.
• POMP is degraded after completion of proteasome maturation, serving as a dedicated assembly factor rather than a permanent subunit.
• Localizes primarily to the cytoplasm and cytosol, but is also found in the nucleus and associated with microsomal membranes.
• Strongly expressed in healthy epidermis from the basal to granular layer; in KLICK patients, expression decreases toward the granular layer, linking it to skin homeostasis and disease.
• Contains an isopeptide bond and undergoes ubiquitin conjugation, reflecting its transient role and regulated turnover during proteasome biogenesis.

Experimental Guidance and Technical Tips

• The immunogen (amino acids 1-141) covers nearly the full-length human POMP protein, suggesting the antibody may recognize native and denatured forms; for Western blot, a band around 16 kDa is expected.
• For WB, consider using reducing conditions and loading positive control lysates from human cell lines (e.g., HeLa, HEK293) that express POMP ubiquitously.
• In ELISA, the antibody can be used for indirect detection; optimizing coating antigen concentration and secondary antibody dilution is recommended for your specific assay format.
• Since POMP is a low-molecular-weight chaperone, ensure adequate transfer times and membrane pore size (0.2 µm PVDF or nitrocellulose) for Western blot.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers a comprehensive supply chain for IVD raw materials, including validated antibody pairs, recombinant proteins, and bulk ancillary reagents. This anti-POMP polyclonal antibody supports research in proteasome biology, protein degradation, and skin-related disorders. As your one-stop sourcing partner, CamelBio can also assist in procuring rare-target raw materials and custom assay development components to accelerate your diagnostic projects.