Anti-POMK Polyclonal Antibody for WB, ELISA - Q9H5K3

Core Product Specifications and Parameters

Biological Background: POMK Function and Localization

• Protein O-mannose kinase (POMK), also known as Sugen kinase 196 (SGK196), is a kinase that specifically phosphorylates the 6-position of O-mannose on the trisaccharide (GalNAc-β1,3-GlcNAc-β1,4-mannose) of α-dystroglycan.
• Phosphorylation is required for dystroglycan to bind extracellular matrix proteins with high affinity, and the enzyme only recognizes the mannose when the disaccharide GalNAc-β3-GlcNAc-β is present, suggesting this motif serves as the substrate recognition element.
• POMK is a type II transmembrane protein anchored in the endoplasmic reticulum membrane, where it exerts its kinase activity co‑translationally on dystroglycan.
• It belongs to the protein kinase superfamily but has an atypical role in carbohydrate modification rather than protein phosphorylation.
• Tissue expression is highest in brain, skeletal muscle, kidney, and heart in both fetal and adult samples, consistent with the tissues most affected in dystroglycanopathies.
• Mutations in POMK cause congenital muscular dystrophy‑dystroglycanopathy type A12 (MDDGA12) and limb‑girdle muscular dystrophy‑dystroglycanopathy type C12 (MDDGC12), characterized by brain and muscle abnormalities.
• The protein migrates with an apparent molecular weight of approximately 40 kDa in SDS‑PAGE.

Experimental Guidance and Technical Tips

• The immunogen is a recombinant fusion protein spanning amino acids 1–350 of human POMK; the polyclonal nature may recognize multiple epitopes and the full‑length protein.
• For Western blotting, a band near 40 kDa is expected in human, mouse, and rat lysates; using tissues with high POMK expression (brain, skeletal muscle) is recommended as positive controls.
• ELISA performance can be optimized by testing serial dilutions of the antibody against the immunogen or cell/tissue lysates.
• As a polyclonal antibody, lot‑to‑lot consistency should be verified when using for quantitative assays.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supports diagnostic developers and research labs with reliable access to IVD raw materials, including this POMK polyclonal antibody for dystroglycanopathy and muscular dystrophy research. In addition to validated primary antibodies like this, CamelBio can supply matched antibody pairs, bulk recombinant proteins, and other ancillary reagents to streamline assay development. Let CamelBio be your sourcing partner for rare‑target raw materials and integrated technical support from concept to clinic.