Anti-POMGNT2 Rabbit Polyclonal Antibody for WB and ELISA - Q8NAT1

Core Product Specifications and Parameters

Biological Background: POMGNT2 Function and Localization

• POMGNT2 (Protein O-linked-mannose beta-1,4-N-acetylglucosaminyltransferase 2), also known as AGO61, GTDC2, or EOGTL, is a glycosyltransferase that catalyzes the transfer of N-acetylglucosamine (GlcNAc) to O-mannose residues on proteins.
• It is essential for the biosynthesis of the phosphorylated O-mannosyl trisaccharide (N-acetylgalactosamine-beta-3-N-acetylglucosamine-beta-4-(phosphate-6-)mannose) on alpha-dystroglycan (DAG1), which is crucial for high-affinity binding to laminin G-like domain-containing extracellular matrix proteins.
• POMGNT2 localizes to the endoplasmic reticulum membrane as a type II transmembrane protein with a signal-anchor, mediating the early steps of O-mannosyl glycan elongation.
• Highly expressed in the brain (cortex and cerebellum), muscle, heart, kidney, and pancreas, reflecting its role in tissues where intact dystroglycan function is critical for cell adhesion and signaling.
• Mutations in POMGNT2 are associated with congenital muscular dystrophy-dystroglycanopathy disorders, including limb-girdle muscular dystrophy and lissencephaly, underscoring its clinical significance in neuromuscular disease.
• The protein has a calculated molecular weight of approximately 67 kDa (UniProt: 66,615 Da; observed: 67 kDa), and the polyclonal antibody recognizes multiple epitopes within the immunogenic region.

Experimental Guidance and Technical Tips

• For Western blot, a band at approximately 67 kDa may be detected under standard reducing conditions; consider optimizing protein loading and transfer for clear visualization.
• To confirm antibody specificity, validate with POMGNT2 knockout or knockdown cell lysates if available, or compare signal with endogenous expression levels across tissues.
• In ELISA, the antibody can be used as a capture or detection reagent; test in direct or indirect formats within the linear range of the assay.
• The immunogen corresponds to amino acids 160-340, which lie within the catalytic domain; ensure that your sample preparation preserves this epitope and consider the possibility of splice variants.

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CamelBio connects diagnostic manufacturers and research laboratories with validated IVD raw materials, including this anti-POMGNT2 polyclonal antibody for glycosylation and muscular dystrophy research. Our comprehensive supply chain supports bulk ordering, optimized antibody pairs, and rare-target sourcing from concept to clinic. Partner with us to streamline your next project with reliable reagents and expert consultation.