Anti-POMC Polyclonal Antibody for WB, IF/ICC, ELISA - P01189

Core Product Specifications and Parameters

Biological Background: POMC Function and Localization

• POMC is a polyprotein precursor cleaved into several bioactive peptides: adrenocorticotropic hormone (ACTH), melanocyte-stimulating hormones (α-MSH, β-MSH, γ-MSH), and β-endorphin.
• ACTH binds to MC2R, stimulating cortisol production and release from the adrenal cortex. Related references: PMID:8636348
• α-MSH and other MSH peptides activate MC1R to promote melanogenesis in skin and hair. Related references: PMID:9620771, PMID:10403794
• Through MC3R and MC4R activation, MSH peptides regulate energy homeostasis and appetite suppression. Related references: PMID:8392067
• POMC-derived peptides also bind MC5R, influencing exocrine gland function.
• POMC is secreted and exhibits tissue-specific expression, notably in the pituitary gland.
• Key post-translational modifications include acetylation, amidation, phosphorylation, and glycosylation.
• β-endorphin acts as an endogenous opioid, contributing to pain modulation and reward pathways.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the N-terminal region (amino acids 1–100) of human POMC; the antibody may detect both full-length POMC and processed fragments that retain this epitope.
• For Western blot, the expected precursor band is near 29 kDa, but smaller immunoreactive bands may be observed in samples with active processing.
• When performing IF/ICC, consider using pituitary-derived cell lines as a positive control and optimize fixation/permeabilization conditions.
• For ELISA, validate the capture-detection pair and sample type to ensure specificity, as POMC levels vary widely across tissues.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and life science researchers with comprehensive access to IVD raw materials, from validated antibody pairs and bulk ancillary reagents to rare-target sourcing. This POMC polyclonal antibody supports neuroendocrine, obesity, and stress research applications, and we can assist with custom polyclonal or monoclonal antibody development, optimized reagent panels, and scalable supply logistics. Contact our team to streamline your diagnostic and discovery workflows.