Anti-POLRMT Rabbit Polyclonal Antibody for WB, IF/ICC, IP, ELISA - O00411

Core Product Specifications and Parameters

Biological Background: POLRMT Function and Localization

• The POLRMT gene encodes the DNA-directed RNA polymerase of the mitochondrial matrix, which is essential for transcribing the mitochondrial genome into RNA using nucleoside triphosphates as substrates. Related references: PMID:21278163, PMID:33602924
• As part of the mitochondrial transcription initiation complex, POLRMT associates with TFB2M and TFAM to enable promoter recognition and opening for basal transcription of mitochondrial DNA. Related references: PMID:29149603
• POLRMT also possesses DNA primase activity, synthesizing short RNA primers required for lagging-strand DNA replication from the origin of light-strand synthesis (OriL). Related references: PMID:18685103, PMID:33602924
• The enzyme localizes to the mitochondria, consistent with its role in mitochondrial gene expression.
• POLRMT belongs to the DNA-directed RNA polymerase family and contains a transit peptide for mitochondrial import.
• Mutations in POLRMT are associated with primary mitochondrial disease (e.g., COXPD55), highlighting its clinical significance.

Experimental Guidance and Technical Tips

• The immunogen corresponds to a recombinant fragment spanning amino acids 42–230 of human POLRMT; the polyclonal antibody is expected to recognize full‑length POLRMT, but epitope mapping is recommended for novel applications.
• For Western blot, the predicted molecular weight is approximately 139 kDa; use an appropriate SDS‑PAGE gel (e.g., 6–8%) and validate the band in your experimental system.
• In immunofluorescence (IF/ICC), expect a mitochondrial staining pattern; co‑staining with a mitochondrial marker is advised.
• For immunoprecipitation (IP), confirm antibody performance under native conditions; pre‑clearing and proper controls are essential.
• The antibody has been tested on human and rat samples; cross‑reactivity with other species (e.g., mouse) has not been reported; validate if needed.
• Always include appropriate positive and negative controls, and titrate the antibody for each application.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supports researchers and diagnostic developers in the mitochondrial biology and transcription fields with a comprehensive portfolio of validated antibodies and ancillary reagents. For your POLRMT workflow, we can supply bulk quantities of this polyclonal antibody, matched antibody pairs, or custom monoclonal alternatives, backed by reliable, documented raw materials. Our one‑stop sourcing model also covers technical consulting and rare‑target procurement, helping you accelerate assay development from concept to clinic.