Anti-POLR3K Rabbit Polyclonal Antibody for WB, ELISA - Q9Y2Y1

Core Product Specifications and Parameters

Biological Background: POLR3K Function and Localization

• DNA-directed RNA polymerase III subunit RPC10 (also known as POLR3K, RPC10, RPC11, RPC12.5) is a core component of RNA polymerase III (Pol III) (PubMed:20413673, PubMed:30584594, PubMed:33335104).
  Related references: PMID:20413673, PMID:30584594, PMID:33335104

• Pol III synthesizes small non-coding RNAs and plays a crucial role in transcription fidelity by mediating proofreading and cleavage of mis-incorporated nucleotides (PubMed:33335104, PubMed:33558764, PubMed:33558766).
  Related references: PMID:33335104, PMID:33558764, PMID:33558766

• Upon reaching the poly(dT) termination signal, POLR3K induces Pol III clamp opening and transcription termination (PubMed:33335104, PubMed:33558764, PubMed:33558766).
  Related references: PMID:33335104, PMID:33558764, PMID:33558766

• POLR3K functions as a nuclear and cytosolic DNA sensor involved in the innate immune response; it senses non-self dsDNA from intracellular bacteria and DNA viruses, leading to transcription into dsRNA that triggers type I interferon and NF-kappa-B pathways via RIG-I (PubMed:19609254, PubMed:19631370).
  Related references: PMID:19609254, PMID:19631370

• Subcellular localization is predominantly nuclear.

• Contains zinc-finger motifs and is a metal-binding protein, essential for its catalytic activity.

• Mutations in POLR3K are associated with leukodystrophy and neurodegeneration, underscoring its importance in neurological health.

Experimental Guidance and Technical Tips

• The immunogen is a recombinant fusion protein covering amino acids 1-108 of human POLR3K (NP_057394.3), which encompasses the full-length protein. This design should yield antibodies that recognize both the native and denatured forms, supporting WB and ELISA applications.
• For Western blot, consider using human cell lysates (e.g., HeLa, HEK293) as positive controls. The predicted molecular weight is 12 kDa, but endogenous expression levels may vary; optimization of primary antibody dilution and incubation conditions is recommended.
• When performing ELISA, validate the antibody with the immunogen or peptide to establish a standard curve. Cross-reactivity with other species has not been tested, so confirm specificity in your model system.
• As a polyclonal antibody, batch-to-batch variability may occur; we recommend including internal controls and performing lot-specific validation for critical experiments.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your comprehensive one-stop sourcing partner for IVD raw materials, supporting diagnostic manufacturers, labs, and research institutes from concept to clinic. For researchers studying RNA polymerase III, transcription regulation, or innate immunity, CamelBio provides reliable access to validated polyclonal antibodies like this anti-POLR3K antibody, along with monoclonal antibodies, bulk ancillary reagents, and custom sourcing for rare targets. Streamline your procurement process by obtaining all critical raw materials through a single, expert-driven channel.