Anti-POLR3C Polyclonal Antibody for WB, ELISA - Q9BUI4

Core Product Specifications and Parameters

Biological Background: POLR3C Function and Localization

• DNA-directed RNA polymerase III subunit RPC3 (also known as RPC62 or POLR3C) is a specific peripheric component of the RNA polymerase III (Pol III) complex, which is responsible for synthesizing small non-coding RNAs, including 5S rRNA, tRNAs, snRNAs, and miRNAs from at least 500 distinct genomic loci. Related references: PMID:20413673, PMID:33558764, PMID:33558766

• As part of the POLR3C/RPC3-POLR3F/RPC6-POLR3G/RPC7 heterotrimer, it coordinates the dynamics of the Pol III stalk and clamp modules during the transition from the apo to the elongation state, which is crucial for efficient transcription initiation and elongation. Related references: PMID:33558764, PMID:33558766

• Beyond its transcriptional role, POLR3C functions as a nuclear and cytosolic DNA sensor in innate immunity. It can sense non-self dsDNA and transcribe it into dsRNA, which subsequently induces type I interferon and NF-κB through the RIG-I pathway, thereby contributing to antiviral defense. Related references: PMID:19609254, PMID:19631370

• POLR3C preferentially binds single-stranded DNA (ssDNA) in a sequence-independent manner, which may facilitate its DNA sensing and polymerase functions. Related references: PMID:21358628

• The protein is localized in the nucleus, consistent with its primary role in transcription.

• POLR3C is a phosphoprotein, and its post-translational modifications may regulate its activity and interactions within the Pol III complex and immune signaling pathways.

Experimental Guidance and Technical Tips

• The immunogen covers the N-terminal region (amino acids 1-270) of human POLR3C, a domain likely conserved across species, which supports the observed cross-reactivity with mouse and rat. For applications in these species, validation in the relevant sample system is recommended.
• In Western blotting, the expected band appears at approximately 61 kDa. Due to potential post-translational modifications or isoform variations, slight shifts in molecular weight may occur; therefore, proper loading controls and molecular weight markers should be included.
• For ELISA, the antibody can be used as a detection reagent; however, optimal dilutions and conditions should be determined empirically using appropriate positive and negative controls.
• As a polyclonal antibody, this reagent recognizes multiple epitopes, which may enhance signal robustness in detection assays. Nonetheless, lot-to-lot consistency should be verified for critical quantitative applications.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, research laboratories, and institutes with comprehensive access to IVD raw materials for transcription and innate immunity research. Our portfolio includes validated antibody pairs, optimized polyclonal antibodies like anti-POLR3C, and bulk ancillary reagents. We also facilitate the sourcing of rare target raw materials, helping to streamline your assay development from concept to clinic.