Anti-POLR2L Polyclonal Antibody for WB, IHC-P, ELISA - P62875

Core Product Specifications and Parameters

Biological Background: POLR2L Function and Localization

• POLR2L encodes the RPABC5 subunit, also known as RPB10 homolog, a shared component of DNA-directed RNA polymerases I, II, and III.
• This subunit is integral to DNA-dependent RNA transcription, using ribonucleoside triphosphates to synthesize ribosomal RNA precursors, mRNA precursors, various non-coding RNAs, and small RNAs like 5S rRNA and tRNAs.
• RPABC5 localizes to the nucleus and nucleolus, consistent with its role in coordinating multiple RNA polymerase activities.
• The protein binds zinc and is classified as a metal-binding transcription factor, contributing to polymerase structural integrity.
• As a member of the reference proteome, POLR2L is highly conserved and essential for fundamental gene expression processes.

Experimental Guidance and Technical Tips

• The immunogen spans full-length human POLR2L (amino acids 1–67), so the antibody is likely to recognize the native 8 kDa protein in Western blotting; consider using low-molecular-weight markers and optimizing transfer conditions.
• For IHC-P, validate nuclear/nucleolar staining patterns in control tissues to confirm specificity.
• Cross-reactivity with mouse and rat enables interspecies studies, but verify expression levels and localization in your model system.
• In ELISA, titrate the antibody to determine optimal working concentration and assess batch-to-batch consistency.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your one-stop IVD raw-material partner, providing validated antibody pairs, optimized polyclonal and monoclonal antibodies, bulk ancillary reagents, and expert sourcing for rare targets. This anti-POLR2L antibody supports development of diagnostic assays and transcriptional research, complementing our comprehensive catalog of polymerase subunit reagents and custom procurement services.